OM-APS复方及其组分对新城疫疫苗免疫效果的影响  

Effects of oxymatrine-astragalus polysaccharide compound and their components on immune response to newcastle disease vaccine in chicken

作　　者：邢玉娟[1] 陈玉库[1] 胡元亮[2] 王德云[2] 陈茜[2] 郭振环[2] 王君敏[2] 赵晓娜[2] 

机构地区：[1]江苏畜牧兽医职业技术学院,江苏泰州225300 [2]南京农业大学中兽医学研究室,江苏南京210095

出　　处：《江苏农业学报》2013年第5期1096-1101,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2008BADB4B06);江苏青蓝工程骨干教师资助项目

摘　　要：为观察OM-APS（氧化苦参碱-黄芪多糖）及其组分对新城疫疫苗免疫效果的影响,将14日龄非免疫健康罗曼蛋公鸡660羽随机均分为11组,第1～9组分别为免疫＋OM-APS、OM和APS高、中、低剂量药物组,第10组为免疫对照组,第11组为不进行免疫和药物处理的空白对照组.除空白对照组外均用NDV-Ⅳ系疫苗滴鼻、点眼免疫,28日龄二免.在首次免疫的同时,1～9组分别口服药物,疫苗对照组和空白对照组口服等量生理盐水,每天1次,连续3d.分别于14、21、28、35和42日龄采血,用MTT法测定T淋巴细胞增殖的变化,用β-微量法测定血清抗体效价变化.于14、28、42日龄采血分离血清,用ELISA方法检测IL-2和IFN-γ的浓度.结果表明：与疫苗对照组比较,高、中剂量的OM-APS、OM和APS均能显著提高血清抗体效价,促进T淋巴细胞增殖,增加血清IL-2和IFN-γ的含量,OM-APS在雏鸡多个日龄段效果优于OM和APS.以上结果表明,OM和APS经组方后可以提高新城疫疫苗的免疫效果.In order to figure out the effects of OM-APS （oxymatrine-astragalus polysaccharide） compound and their components on immune response of ND （newcastle disease） vaccine, six hundred and sixty 14-day-old non-immunized Roman cocks were randomly divided into 11 groups. Groups 1 to 9 were treated with OM-APS, OM and APS at high dose, medium dose, and low dose, respectively, and group 10 was treated with vaccine and group 11 was blank control. Groups 1 to 10 were inoculated with NDV-IV vaccine through intraocular-nasal route, repeated vaccination at day 28. At the same time of the first vaccination, the chickens in groups 1 to 9 were given the compounds through oral administration, and the chickens in group 1 and blank control group were given physiological saline, once a day for three days. On days 14, 21,28, 35 and 42, peripher- al T lymphocyte proliferations and serum antibody titers were determined by MTT method and 13-microscopic method respec-tively. On days 14, 28 and 42, concentrations of IL-2 and IFN-α of serum were determined by double antibody sandwich ELISA method. Tymphocyte proliferation, anti- body titer and cytokines of chickens in the groups treated with high-and medium-dose of OM-APS, OM and APS were obviously higher than those in vaccine control group. Atsame ages of chickens, OM-APS performed better than OM and APS improving immune response to NDV-IV vaccine in chick- en. It was indicated that the immunity of newcastle disease vaccine could be improved by OM-APS compound.

关 键 词：氧化苦参碱-黄芪多糖 淋巴细胞增殖 抗体效价 细胞因子 

分 类 号：S852.52[农业科学—基础兽医学]