低温胁迫对马来西亚红罗非鱼血清生化指标的影响  

Effects of Cooling Temperature Stress on Serum Biochemical Indexes in Malaysian Red Tilapia(Oreochromis mossambicus×O.niloticus)

作　　者：朱文彬[1] 刘浩亮[2] 陈作志[3] 梁政远 董在杰[1,2] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心,农业部淡水渔业和种质资源利用重点实验室,江苏无锡214081 [2]南京农业大学无锡渔业学院,江苏无锡214081 [3]中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东广州510300 [4]无锡瑞顺水产养殖科技有限公司,江苏无锡214043

出　　处：《水产学杂志》2013年第5期16-20,共5页Chinese Journal of Fisheries

基　　金：农业部南海渔业资源开发利用重点实验室开放课题资助(LSF2001-02);中央级公益性科研院所基本科研业务费专项资金(2013JBFT06)

摘　　要：在室内自然降温条件下,研究了马来西亚红罗非鱼(Oreochromis mossambicus×O.niloticus)致死低温,检测了在温室越冬温度(17.8℃)和低温胁迫下开始死亡时红罗非鱼血清的生化指标。结果表明,当水温降到8.6℃时,马来西亚红罗非鱼开始死亡,死亡低温范围为8.6～6.4℃,半致死低温为7.6℃;8.6℃时,红罗非鱼血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)和肌酸激酶同工酶(CK-MB)5种酶的活性显著高于17.8℃时(P<0.05),肌酐(CREA)含量、K+浓度显著上升(P<0.05),总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO),及Ca2+含量小幅增加,而Na+和Cl-含量均显著下降(P<0.05),胆固醇(CHOL)和甘油三酯(TG)含量略有降低。The lethal temperature was studied in Malaysian red tilapia （Oreochromis mossambicus xO. niloticus） under indoor natural cooling, and serum biochemical indexes were tested at water temperature of 17.8~C and beginning of death in the red tilapia. The re- suits showed that red tilapia death appeared when the temperature dropped to 8.6~C, with range from 8.6~C to 6.4~C, and the LCs0 low temperature of 7.6~C. There were significantly higher activities of ALT, AST, LDH, ALP and CK-MB at 8.6~C compared with at 17.8~C （P〈0.05）. The CREA level and the content ofK＋ were found to be significantly increased （P〈0.05） at 8.6~C, while TP, ALB, GLO and the content of Ca2＋ increased slightly. However, the contents of Na＋ and C1- were significantly decreased （P〈0.05）, with slightly lower CHOL and TG levels.

关 键 词：马来西亚红罗非鱼 低温胁迫 致死低温 血清生化指标 

分 类 号：S917[农业科学—水产科学]