荧光色谱测定纳米粒体系中阿霉素含量  

Determination of the Concentration of Doxorubicin in Nanoparticles by HPLC with Fluorescence Detector

作　　者：吕凤娇[1] 吴洪[1] 许小平[2] 

机构地区：[1]泉州师范学院化学与生命科学学院,福建泉州362000 [2]福州大学化学化工学院,福建福州350002

出　　处：《宜春学院学报》2013年第9期121-124,共4页Journal of Yichun University

基　　金：泉州师范学院重点学科建设项目资助(MDSCh-2009A);福建省教育厅A类科技项目(JA11223)

摘　　要：研究制备以聚乙烯吡咯烷酮为载体的阿霉素纳米粒,提出用HPLC-荧光色谱法测定聚乙烯吡咯烷酮-阿霉素纳米粒体系中阿霉素含量,并采用该方法测定了纳米粒的包封率和载药量.结果表明,制备得到的阿霉素纳米粒粒径小,球形度好；HPLC-荧光色谱法对聚乙烯吡咯烷酮-纳米粒体系中的阿霉素含量测定具有专属性强、方便快捷的特点；阿霉素在0.08～8 μg/mL范围内线性关系良好（R2 =0.9999）,回收率高；阿霉素纳米粒载药量为（2.92±0.09）％,包封率为（83.21±2.17）％.Objective： Doxorubicin- loaded nanoparticles, using doxorubicin （ADM） as model drug, polyvi-nylpyrrolidone （PVP） as matrix material, were prepared in this paper. Methods： More over, the drug loading and encapsulation efficiency were measured by high performance liquid chromatography with fluorescence detection （HPLC -FLD） . Results： The results showed that the nanoparticles exhibited a spherical shape and a small size from the observation of SEM. Calibration curve of doxorubicin in the system was constructed in the concentration range 0. 08 - 8.0 μg/mL （ R2 = 0. 9999 ） . Their drug - loaded amount and encapsulation efficiency were （2. 92 ±0. 09） % and （83.21 ± 2. 17） % respectively. Conclusions： The consequence indicated that the HPLC -FLD was fine to detect the concentration of doxorubicin in nanonarticles.

关 键 词：纳米粒 阿霉素 荧光色谱 

分 类 号：R91[医药卫生—药学]