透骨草提取物对人肝癌SMMC-7721细胞凋亡及凋亡相关因子的影响  

The influences of Phryma Leptostachya L. var. asiatica Hara extract on apoptosis and apoptosis-related factors of human hepatocellular carcinoma SMMC-7721 cells

作　　者：李萍[1] 曾常茜[2] 

机构地区：[1]吉林省图们市人民医院内科,图们133100 [2]大连大学医学院,大连116622

出　　处：《天津中医药》2013年第10期612-614,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：2010年大连市科学技术基金计划项目(2010E13SF144)

摘　　要：[目的]观察透骨草提取物对人肝癌SMMC-7721细胞凋亡的影响,探讨透骨草提取物诱导SMMC-7721细胞凋亡的分子机制。[方法]流式细胞术检测透骨草提取物诱导SMMC-7721细胞凋亡的影响;免疫组化法检测透骨草提取物对SMMC-7721细胞caspase-9、-3、-7和X连锁凋亡抑制蛋白(XIAP)表达的影响。[结果]40.91μg/mL透骨草提取物处理的SMMC-7721细胞凋亡率为(26.84±1.23)%,明显高于对照组(P<0.05)。40.91μg/mL透骨草提取物处理的SMMC-7721细胞Caspase-9、-3、-7蛋白表达明显低于与对照组(P<0.05),而XIAP蛋白表达明显高于对照组(P<0.05)。[结论]透骨草提取物能通过XIAP/Caspase信号通路抑制SMMC-7721细胞凋亡。[Objective] To observe apoptosis of human hepatocellular carcinoma SMMC-7721 cells induced by Phryma Leptostachya L. var. asiatica Hara extract and to investigate the related molecular mechanism. [Methods] Flow cytometry was used to detect the apoptosis rate of SMMC-7721 cells induced by Phryma Leptostachya L.var, asiatica Hara extract, lmmunohistochemistry technique was used to determine the expressions of caspase-9,-3,-7 and XIAP proteins of SMMC-7721 cells. [Results] The apoptotic rate of SMMC-7721 cells treated with 40.91 μg/mL Phryma Leptostachya L. vat. asiatica Hara extract was （26.84±1.23）% and increased obviously than control group （P〈0.05）. The expressions of Caspase-9 ,-3 ,-7 proteins of SMMC-7721 cells treated with 40.91μg/mL Phryma Leptostachya L. vat. asiatica Hara extract decreased than control group （P〈0.05）. The expression of XIAP protein of SMMC-7721 cells treated with 40.91 μg/mL Phryma Leptostachya L. vat. asiatica Hara extract increased than control group （P〈0.05）. [Conclusion] Phryma Leptostachya L. var. asiatica Hara extract could induce the apoptosis of SMMC-7721 cells by XIAP/Caspase signal pathway.

关 键 词：透骨草提取物 SMMC-7721细胞 凋亡 CASPASE-9 

分 类 号：R285.5[医药卫生—中药学]