抵当芪桂汤对STZ诱导糖尿病大鼠血浆TXB_2和血小板表面CD62_P、CD63的影响  

Effects of Didang Qigui Decoction on blood plasma TXB_2 and platelet surface CD62_P/ CD63 in STZ-induced diabetes rats

作　　者：赵天才[1] 杨景锋[1] 任艳芸[2] 张永宜[1] 刘飞飞 

机构地区：[1]陕西中医学院中医临床医学,咸阳712046 [2]陕西中医学院附属医院肾病科 [3]河南省洛阳新区人民医院内一科

出　　处：《环球中医药》2013年第9期658-660,共3页Global Traditional Chinese Medicine

基　　金：陕西省自然科学基础研究计划(2011JM4017);陕西省教育厅专项科研计划(11JX06751)

摘　　要：目的观察抵当芪桂汤对糖尿病大鼠血浆血栓素B2(Thromboxane B2,TXB2)含量及血小板表面α颗粒膜糖蛋白(alpha-granular membrane glycoprotein,CD62p)和血小板溶酶体膜蛋白(lysosome intact membrane protein,CD63)表达的影响。方法 70只大鼠按随机数字表法抽取10只为空白组,其余大鼠用链脲佐菌素(streptozocin,STZ)复制糖尿病模型,选取成模大鼠按随机数字表法分为模型组、格列美脲组、抵当芪桂汤大量组、抵当芪桂汤常量组,给予相应干预10周后,测定血浆TXB2含量及血小板表面CD62P、CD63的表达。结果干预10周后,与E组比较,B组、C组、D组大鼠血浆TXB2含量及血小板表面CD62P、CD63表达率明显降低,有显著性差异(P<0.05)。结论抵当芪桂汤可通过降低糖尿病大鼠血浆TXB2含量和血小板表面CD62P、CD63表达率,抑制血小板活化、改善微循环而阻止或延缓糖尿病血管病变的发生和发展。Objective To observe therapeutic effects of Didang Qigui Decoction upon rats with diabetes that is induced by streptozotiein（STZ） by examining the levels of TXB2 in blood plasma and granule membrane glycoprotein（CD62P）, lysosomal interal membrane protein （CD63） in platelet surface. Method 10 out of 70 female rats are chosen randomly as the blank control group（group A）, then the rest are induced by STZ, waiting to develop diabetes rats which, afterwards, are divided randomly into model group （group B） , glimepiride group（ group C） , maximum dosage group of the Decoction（ group D）, and Didang Qigui Decoction normal-dose group（group E）. 10 weeks intervention is given to all the above groups and the levels of TXB2 in blood plasma and CD62P, CD63 in platelet surface in each group are measured. Result After l0 weeks of intervention, comparing with group E, the levels of TXB2 in blood plasma and CD62p, CD63 in platelet surface were decreased in group B, group C and group D （P 〈0. 05）. Conclusion It is possible that Didang Qigui Decoction restrains platelet activation and improves microcirculation by reducing blood plasma TXB2 and platelet surface CD62p, and CD63 content and further prevents or delays the occurrence and development of diabetic angiopathy.

关 键 词：糖尿病 抵当芪桂汤 CD62_P CD63 TXB_2 

分 类 号：R285.5[医药卫生—中药学]