饲料铜含量对亚硝酸盐胁迫下斑节对虾血细胞的影响  

Effects of Feed Copper Content on Haemocyte of Penaeus monodon Under Nitrite Stress

作　　者：冼健安[1] 苗玉涛[1] 叶超霞[1] 叶剑敏[1] 郭慧[1] 张胜鹏[1] 王安利[1] 

机构地区：[1]华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,生态与环境科学广东普通高校重点实验室,广州510631

出　　处：《水生态学杂志》2013年第5期71-75,共5页Journal of Hydroecology

基　　金：国家自然科学基金(30671628;31100296);中国博士后科学基金(2012M511829);广东省自然科学基金(S2011020003256;S2012040008093);广东省科技计划项目(2011B020307010;2012B020307004)

摘　　要：研究了饲料铜离子含量对亚硝酸盐胁迫下斑节对虾（Penaeusmonodon）血细胞参数的影响。向斑节对虾投喂不同铜离子添加量（0、10、25、40、55、110mg／kg）的饲料8周后，进行24h的20mg／L亚硝酸氮胁迫，取样测定对虾的血细胞总数（THC）、血细胞活性氧（ROS）含量和血细胞凋亡率。结果显示，经亚硝酸盐胁迫后，25—55mg／kg饲料组对虾能保持较高的THC以及较低的血细胞ROS含量和凋亡率；110mg／kg饲料组对虾的THC最低，血细胞ROS含量和凋亡率均最高，研究表明，饲料中添加25—55mg／kg的铜离子能有效降低亚硝酸盐胁迫对血细胞造成的氧化损害，但过量添加（110mg／kg）反而对血细胞造成更为严重的损害。This paper studied the effects of feed copper content on haemocyte parameters of Penaeus monodon under nitrite stress. Feeding these Penaeus monodon with different dose of copper ion contents （0, 10, 25, 40, 55 and 110 mg/kg） for 8 weeks, with the stress of 20 mg/L nitrite for 24 h, it would take samples to determine its total haemocyte count （THC）, haemocyte reactive oxygen species （ROS） content and haemocyte apoptotic ratio. The results showed that the feed group, which fed with copper content of 25 - 55 mg/kg, would keep higher THC, low- er haemocyte ROS content and lower apoptotic ratio. The feed group, which fed with copper content of 110 mg/kg, would keep the lowest THC, the highest ROS feeding with copper content of 25 -55 mg/kg by nitrite stress. However, excessive feeding haemoeytes. content and the highest apoptotic ratio. These results indicated that could effectively reduce the oxidative damage for haemocytes caused with copper content of 110 mg/kg would exacerbate the damage on haemocytes.

关 键 词：亚硝酸盐 铜 斑节对虾 血细胞 氧化胁迫 

分 类 号：S963.7[农业科学—水产养殖]