烟梗废料生物脱胶工艺条件的优化  

Optimization of Conditions for Reducing Pectin in Waste Tobacco Stem with Pectinase

作　　者：王静[1] 李永宽 李坤[1] 王中杰[1] 王姣[1] 刘慧[1] 陈彦好[1] 朱大恒[1] 

机构地区：[1]郑州大学生物工程系,郑州市科学大道100号450001 [2]河南中烟工业有限责任公司郑州卷烟厂,郑州市陇海东路72号450004

出　　处：《烟草科技》2013年第10期59-62,共4页Tobacco Science & Technology

基　　金：中国烟草总公司河南省公司科技项目"烟梗废料发酵生产燃料乙醇和生物树脂的研究"(HYKJ201021);郑州大学大学生创新创业训练计划资助项目"基于代谢调控的深红酵母发酵废烟梗产类胡萝卜素响应面优化研究"(2012cxsy107)

摘　　要：为降低烟梗果胶含量,提高烟梗在再造烟叶加工中的可用性和再造烟叶的品质,以烟梗废料为材料,采用正交实验对烟梗的生物脱胶工艺进行了优化。结果表明:以果胶去除率为指标,烟梗的果胶酶脱胶最佳工艺条件为:酶解温度45℃、酶解时间8 h、溶液pH 5.8、每10 g烟梗果胶酶用量为0.2 g。此条件下果胶去除率达到81.02%。For improving stem＇s usability in reconstituted tobacco production and raising reconstituted tobacco quality, the pectin in tobacco stem was reduced with pectinase and the reaction conditions were optimized with orthogonal experiments. The results showed that from the point of pectin removal rate, the optimum reaction conditions were： reaction temperature 45 ℃ for 8 hours, pH of solution 5.8 and 0.2 g of pectinase for every 10 g of tobacco stem. Under such conditions, pectin removal rate was up to 81.02%.

关 键 词：烟梗废料 生物脱胶 果胶酶 

分 类 号：TS452.6[农业科学—烟草工业]