雄蚕四元杂交组合的不完全双列杂交分析  

The Incomplete Diallel Analysis of Male Silkworm Tetra-crossing Combination

作　　者：陈诗[1] 祝新荣[1] 孟智启[1] 柳新菊[1] 何克荣[1] 

机构地区：[1]浙江省农业科学院蚕桑研究所,杭州310021

出　　处：《蚕业科学》2013年第5期900-905,共6页ACTA SERICOLOGICA SINICA

基　　金：浙江省农业新品种选育重大科技专项(No.2012C12910)

摘　　要：优化雄蚕多元杂交组合的组配方式可以增强其杂种优势。选用12个中系家蚕斑纹限性杂交原种和12个日系家蚕性连锁平衡致死系杂交原种,按不完全双列杂交的形式组配成144个雄蚕四元杂交组合。先依据不完全双列杂交试验分析各杂交组合的结茧率、死笼率、虫蛹率、全茧量、茧层量、茧层率、万蚕产茧量和万蚕茧层量8项主要经济性状的一般配合力(GCA)、特殊配合力(SCA)和相关的遗传参数,然后依据雄蚕四元杂交组合只能利用雄性性连锁平衡致死系与雌性普通品种杂交的特点,构建雄蚕四元杂交组合不完全双列杂交模型。应用该模型进一步分析以上8项经济性状在单品系(祖亲)的一般配合力,筛选出具有较好单品系一般配合力效应值的母本品种蒙菁、限菁和云7,父本品种平30、平60和平72。上述8项经济性状的遗传参数分析结果以及构建的不完全双列杂交模型对雄蚕四元杂交组合的选配具有一定的实用指导意义。Optimizing combination of multiple hybrids of male silkworm can strengthen its heterosis. Parent eggs of 12 Chinese strain silkworm varieties with sex-limited marks and 12 Japanese strain silkworm sex-linked balanced lethal lines were used to prepare 144 tetra-crossing combinations of male silkworm by using the form of incomplete diallel crossing. General combining ability （GCA）, special combining ability （SCA） and related genetic parameters of 8 main economic characters （cocooning rate, rate of dead cocoon, larva-pupa rate, cocoon weight, cocoon shell weight, cocoon shell rate, cocoon weight per 10 000 larvae, and cocoon shell weight 10 000 larvae） were analyzed with incomplete diallel crossing test. Then, based on the character that tetra-crossing combination of male silkworm can only be prepared from crossing between male silkworm of sex linked balanced lethal line and female silkworm of common strain, an incomplete diallel cross model for tetra-crossing combination of male silkworm was constructed. Through further analyses on general combining ability of the above 8 main economic characters of single line （grandparent） by this model, maternal lines Mengqing, Xianjing and Yun 7 and paternal lines Ping 30, Ping 60 and Ping 72 with high single line GCA value were ob- tained. The genetic parameter analysis of these 8 main economic characters and the constructed incomplete diallel cross model provide certain guidance to the breeding of male silkworm tetra-crossing combination.

关 键 词：雄蚕 不完全双列杂交 四元杂交组合 配合力 

分 类 号：S882.2[农业科学—特种经济动物饲养]