益肝解毒方对非酒精性脂肪性肝炎大鼠血清MDA、SOD及IR的影响  

Effect of “Yigan Jiedu Decoction”on serum MDA,SOD and insulin resistance in rats with nonalcoholic fatty steatohepatitis

作　　者：郑昕[1] 王亚平[1] 张志银[1] 朱艳[1] 庄敏之[1] 张迪[1] 周菲[1] 韩静[1] 郭兆玮[1] 吴瑛[1] 

机构地区：[1]上海交通大学附属第一人民医院分院中医脂肪肝病专科,上海200081

出　　处：《上海中医药杂志》2013年第10期66-69,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市虹口区科委医学立项课题资助项目(1102-02)

摘　　要：目的通过高脂饮食建立非酒精性脂肪性肝炎(NASH)大鼠模型,观察益肝解毒方对其血清生化和肝组织病理的影响。方法将40只SD大鼠随机分为正常组、模型组、中药组、西药组,模型组、中药组、西药组给予高脂饲料喂养12周以复制非酒精性脂肪性肝炎模型,中药组以中药灌胃,西药组以易善复(多烯磷脂酰胆碱)灌胃,疗程8周。20周后处死大鼠,收集标本。测定各组大鼠体质量、肝重、肝体比以及血清三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、谷氨酰转肽酶(GGT)、血清丙二醛(MDA)、超氧化物歧化酶(SOD)、空腹血糖(FBG)、胰岛素(FINS)水平,并通过HOMA-IR法计算胰岛素抵抗指数(IR);同时对肝组织进行HE染色,观察其病理。结果大鼠NASH模型成功建立。与模型组比较,中药组大鼠的肝体比、血清ALT、AST、GGT、FBG、MDA明显下降,差异有统计学意义(P<0.05),血清SOD明显升高,差异有统计学意义(P<0.05);与对照组比较,中药组大鼠胰岛素抵抗指数明显降低,差异有统计学意义(P<0.01);其肝细胞病理显示炎症活动程度明显改善。结论益肝解毒方对高脂饮食诱导的NASH大鼠的肝功能具有良好的改善作用,且能改善胰岛素抵抗状态。Objective The rat models of non-alcoholic steatohepatitis（NASH） were established by high fat diet. To observe the clinical efficacy of ＂Yigan Jiedu Decoction＂ on serum biochemistry and hepatic tissue. Methods The 40 SD rats were randomly divided into normal group, model group, Chinese medicine group and western medicine group. The rats in nmdel group, Chinese medicine group and western group were fed with high-fat diet for 12 weeks to duplicate the models. Rats in Chinese medicine were treated with Chinese medicine, and rats in western group were treated with polyene phosphatidyl choline, with the course of 8 weeks. All rats were sacrificed at the 20th week to collect the samples. The body weight, hepatic wet weight, ratio of liver weight and body weight, serum TG, TC, HDL, LDL, ALT, AST, GGT, MDA and SOD were determined. Homeostasis model assessment was applied to assess the status of insulin resistance（ HOMA- IR）. Hepatic tissues were detected by HE staining. Results Non-alcoholic steatohepatitis in the rat model was successfully established. Compared with model group,trcaUnent group on the rats of NASH induced by a high-fat diet had lower ratio of liver weight and body weight ,lower serum levels of ALT,AST, GGT, FBG, MDA and higher serum levels of SOD（P 〈 0.05）. Compared with polyene phosphatidyl choline, yigan jiedu decoction could improve the insulin resistance index and the results had statistics significance （P 〈 0.01 ）. Serum levels of TC,TG, LDL, HDL had a downward trend during the experimental period, but its distinction did not prove a statistical significance（ P 〉 0.05 ）. Liver pathology showed inflammatory activity was significantly improved in rats of treatment group. Conclusion ＂Yigan Jiedu Decoction＂ can impreve the liver function and insulin resistance in rats with NASH induced by high-fat diet.

关 键 词：非酒精性脂肪性肝炎 益肝解毒方 实验研究 

分 类 号：R285.5[医药卫生—中药学]