灵杆菌多糖对内毒素诱导小鼠肺损伤的保护作用  

The protective role of hemarisin in endotoxin-induced pulmonary damage in mice

作　　者：张群[1] 汪燕[1] 余传林[2] 陈娜娜[2] 雷林生[2] 

机构地区：[1]南方医科大学第三附属医院,广州市510515 [2]南方医科大学药学院,广州市510515

出　　处：《实用医学杂志》2013年第20期3298-3300,共3页The Journal of Practical Medicine

基　　金：广东省自然科学基金(编号:7005189);广东省医学基金(编号:B2012246)

摘　　要：目的:探讨灵杆菌多糖对内毒素(LPS)所致小鼠肺损伤的保护作用。方法:提前0.5 h给小鼠腹腔注射灵杆菌多糖,然后尾静脉注射LPS(30 mg/kg),3 h后ELISA法测定肺组织白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的含量;6 h后测量肺湿重干重比(W/D)、髓过氧化物酶(MPO)活力、丙二醛(MDA)含量。结果:灵杆菌多糖(100 U/kg)可部分抑制由LPS引起的IL-1β、IL-6、TNF-α的增加(P<0.05),降低肺W/D(P<0.05);剂量为100、50 U/kg时,可部分抑制MPO活性(P<0.05)及MDA含量(P<0.01)的增加。结论:灵杆菌多糖预防性给药对内毒素所致小鼠肺损伤有一定的保护作用。Objective To investigate the protective role of hemarisin （lipopolysaccharides from Bacillus prodigiosus） in endotoxin （LPS）-induced pulmonary damage in mice. Methods 0.5 hours after intraperitoneal injections of hemarisin, the mice received LPS of 30 mg/kg via the tail vein. 3 hours after injections of LPS, levels of interleukin-lβ （IL-1β）, interleukin-6 （IL-6）, and tumor necrosis factor α （TNF-α） in the lung tissues were detected by ELISA. 6 hours later, the lung wet weight and dry weight ratio （W/D）, myeloperoxidase （MPO） activity, and malondialdehyde （MDA） content were determined. Results Hemarisin of 100 U/kg partially inhibited a LPS-induced increase in IL-1β, IL-6, and TNF-α （P 〈 0.05） and decreased the W/D ratio （P 〈 0.05）. Hemarisin at a dose of 100 or 50 U/kg partially suppressed MPO activity （P 〈 0.05） and an elevation in MDA content （P 〈 0.01 ）. Conclusions Prophylactic administration of hemarisin plays a certain role in the protection of endotoxin-induced pulmonary damage in mice.

关 键 词：灵杆菌多糖 内毒素 肺损伤 髓过氧化物酶 丙二醛 促炎细胞因子 

分 类 号：R285.5[医药卫生—中药学]