发育性髋关节脱位早期髋臼软骨细胞凋亡与Bcl-2、Bax表达的相关性研究  

Relationship between the expressions of Bcl-2 and Bax and acetabular cartilage cell apoptosis of early developmental dislocation of the hip

作　　者：王炳海[1] 韦宜山[1] 丁良甲[1] 边鸿飞 崔兆鹏[1] 王海瑞[1] 

机构地区：[1]内蒙古医科大学第二附属医院,呼和浩特市010030

出　　处：《实用医学杂志》2013年第20期3301-3303,共3页The Journal of Practical Medicine

摘　　要：目的:探讨发育性髋关节脱位(DDH)早期髋臼软骨是否发生了过度的细胞凋亡,以及Bcl-2、Bax的表达与髋臼软骨细胞凋亡发生的关系。方法:选取新西兰大白兔40只,雌雄不限,4周龄,体重(0.5±0.05)kg,平均0.5 kg,采用兔后肢伸膝屈髋位管型石膏固定方法制作DDH模型。将左后肢管型石膏固定为实验侧,右后肢不做处理为空白对照侧。结果:DDH造模成功率60%(24/40),证实DDH早期髋臼软骨中存在过度的细胞凋亡,Bcl-2在髋臼软骨中的表达与Bax的表达结果相反,且Bcl-2/Bax比值与髋臼软骨细胞凋亡率呈正相关。结论:DDH早期髋臼软骨细胞凋亡及凋亡相关因子可能是髋臼软骨退变的重要因素之一,并参与了DDH的髋臼发育不良的发生和发展。Objective To investigate whether there was excessive apoptosis of aeetabular cartilage cell in early stage of developmental dislocation of the hip （DDH）, and to explore the relationship of the expressions of Bcl-2 and Bax with acetabular cartilage cell apoptosis. Methods 40 New Zealand white rabbits I male or female, 4 weeks old, （0.5 ± 0.05）kg] were included to produce DDH model by fixing rabbit right hindlimb to stretched knee and flexing hip with plaster east models. The left hind limb was plaster casted as experimental group, and the right hind limbs which were not treated served as control group. Results The successful rate of DDH modeling was 60%, and excessive apoptosis of acetabular cartilage cells did exist in early stage of DDH. The expressions of Bax and Bcl-2 in aeetabular cartilage were opposite, and the ratio of Bel-2/Bax and the rate of acetabular cartilage apoptosis were positively correlated. Conclusion Apoptosis related factors and excessive apoptosis of acetabular cartilage cells in early stage of DDH may be one of the important factors affecting the degeneration of aeetabular cartilage, and may involve in the occurrence and development of acetabular dysplasia in DDH.

关 键 词：髋脱位 软骨细胞 细胞凋亡 Bcl-2 BAX 

分 类 号：R684[医药卫生—骨科学]