多重耐药鲍曼不动杆菌医院感染同源性分析  

Homology analysis on Multidrug-resist Acinetobacter baumannii

作　　者：方艳平[1] 张耀康[2] 江凌晓[1] 姜长宏[1] 林丽娟[1] 付亮[1] 龙军[1] 

机构地区：[1]南方医科大学珠江医院检验医学部,广州市510280 [2]南方医科大学2009级检验系,广州市510515

出　　处：《实用医学杂志》2013年第20期3409-3411,共3页The Journal of Practical Medicine

基　　金：广东省科技攻关项目(编号:c1211220700228)

摘　　要：目的:对多重耐药鲍曼不动杆菌(multiple drug resistance acinetobacter baumannii,MDR-Ab)同源性进行分析,为临床合理治疗MDR-Ab引起的感染和控制院内感染提供依据。方法:对某医院脑外科重症病房分离的12株MDR-Ab,进行脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)分析其同源性。结果:12株MDR-Ab试验菌株中,经PFGE指纹图谱分析,可分为A、B、C、D、E、F 6个基因型,其中A型和B型是主要流行基因型。结论:该研究证明该院脑外科患者感染的鲍曼不动杆菌呈多克隆系并存,但尚不能证明存在鲍曼不动杆菌医院感染爆发流行,PFGE是研究临床菌株流行,菌株分型的有效手段。Objective multidrug-resistant Acinetobacter baumannii （MDR-Ab） strains were typed by Pulsed-field gel electrophoresis （PFGE）, and to provide the basis for the clinical treatment of infection from MDR-Ab and to control nosocomial infection. Methods The DNA of 12 strains of Acinetobacter baumannii isolated from the Intensive Care Unit of Neurosurgieal Department was analyzed by （MDR-Ab）. Results According to PFGE fingerprint analysis, the DNA of 12 strains of Acinetobacter baumannii can be divided into six genotypes of A, B, C, D, E, F, and genotypes of A and B are the main genetic type. Conclusion The coexistence of multiple cloning system in this was proved by PFGE, but the outbreak of the Nosocomial infection was not proved yet .furthermore, PFGE is an effective way for studying epidemiology.

关 键 词：多重耐药 鲍曼不动杆菌 脉冲场凝胶电泳 院内感染 

分 类 号：R446.5[医药卫生—诊断学]