三仁汤对脾胃湿热证小鼠Aquaporin-3的影响  

Effects of Sanren decoction on kidney Aquaporin-3 in mice with spleen-stomach-damp-heat syndrome

作　　者：万滢聪 吴泽徐 翁雪荣 涂肖仪 李志勇[1] 文小敏[1] 陈小玲[1] 陈景良[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515

出　　处：《时珍国医国药》2013年第10期2348-2350,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家中医药管理局中医药科学技术研究专项(No.06-07JP33)

摘　　要：目的研究三仁汤对脾胃湿热证小鼠肾组织AQP3变化。方法将30只SPF级BACB/C小鼠随机分为正常对照组、脾胃湿热模型组、三仁汤治疗组,测量小鼠体温、体重,测定肾组织AQP3的水平,病理切片观察小鼠肾脏病理变化。结果与正常对照组相比,脾胃湿热模型组体温偏高,体重增长缓慢,AQP3含量显著升高,差异有统计学意义(P<0.05),病理提示肾脏存在炎症损伤,三仁汤治疗组AQP3稍升高,与正常对照组相比差异无统计学意义(P>0.05),与脾胃湿热模型组相比差异有统计学意义(P<0.05),病理提示三仁汤对肾脏的炎症损伤有修复作用。结论脾胃湿热证小鼠肾组织AQP3呈高表达状态,三仁汤能调节脾胃湿热证小鼠肾脏AQP3的表达,恢复机体正常的水液代谢平衡。Objective To investigate the effects of Sanren decoction on kidney AQP3 in mice with spleen - stomach - damp - heat （DHSS） syndrome. Methods 30 SPF BACB/C mice were randomly divided into normal control group, DHSS model group and Sanren decoction treatment group, with the same number of male and female. The effects of decoction on the body mass and body temperature, water and food intake, mental status and feces , AQP3 level and histopathological changes of kidney were meas- ured and evaluated. Results The DHSS model group grew less slowly than the normal control group, with less water and food in- take, and higher body temperature. The kidney AQP3 level in the DHSS model group significantly increased compared with the nor- mal control group （ P 〈 0.05 ）. The pathological section of the DHSS model group revealed renal inflammatory lesions. The kidney AQP3 level in the Sanren decoction treatment group slightly increased compared with those in the normal control group （ P 〉 0. 05 ）. [t was statistically significant in the kidney AQP3 level between the Sanren decoction treatment group and the DHSS model group （ P 〈 0.05 ）. The pathological section revealed that Sanren decoction treatment group made renal inflammatory lesions recov- er to a certain extent. Conclusion The expression of kidney AQP3 is in a high state in mice with DHSS syndrome. Sanren decoc- tion resumes the body water metabolism balance by regulating the expression of kidney AQP3.

关 键 词：脾胃湿热证 三仁汤 水孔蛋白类(AQP3) 

分 类 号：R285.5[医药卫生—中药学]