不同电针刺激频率和频度对小鼠免疫功能的作用  

The effect of electroacupuncture with different frequencies and number of stimuli on immune function in mice

作　　者：胡志苹[1] 黄诚[1] 

机构地区：[1]赣南医学院,江西赣州341000

出　　处：《时珍国医国药》2013年第10期2538-2541,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.31060144);江西省自然科学基金(No.2008GZY0067)

摘　　要：目的在小鼠上探讨不同电针(EA)刺激频率和频度对其免疫功能的作用。方法应用细胞生物学和MTT方法,检测不同电针刺激频率和频度对小鼠脾指数、NK细胞活性和T细胞增殖能力等免疫学指标的影响。结果电针1次后,分别在0,24和48h时间点检测小鼠的脾指数、脾脏NK细胞活性及T细胞增殖能力,2 Hz、100 Hz和2/100 Hz EA刺激均无明显影响。连续电针3 d(每天1次)后,2Hz、100Hz和2/100 Hz EA对脾指数均无明显影响;100Hz EA显著降低NK细胞活性(P<0.05),2/100 Hz EA明显升高NK细胞活性(P<0.05),而2Hz EA无明显影响;2Hz EA显著升高脾脏T细胞增殖能力(P<0.01),而100Hz和2/100 Hz EA均无明显影响。结论电针对小鼠脾指数、NK细胞活性和T细胞增殖能力的作用存在频率和频度的特异性,这将为临床应用电针治疗与免疫相关疾病提供理论和实验依据。Objective To explore the effect of electroacupuncture （EA） with different frequencies and number of stimuli on im- mune function in mice. Methods Application of cytobiology and MTT method,the effect of EA at different frequency and number of stimuli on spleen index, NK cell activity and T cell proliferative ability were determined in mice. Results After one time of EA, the spleen index, NK cell activity and T cell proliferative ability in mice were detected respectively at 0,24 and 48h time points, the result showed that there was no significant change in the 2 Hz,100 Hz and 2/100 Hz EA. After three days of successive EA with frequency of 2Hz, 100Hz and 2/100 Hz,the splenic index did not significantly changed; NK cell activity significantly reducedwhen treated with 100Hz EA （P 〈 0.05 ）, and significantly increased when applied with 2/100 Hz EA （ P 〈 0.05 ）, while there was no significant change with 2Hz EA on NK cell activity ; Splenic T cell proliferative ability was significantly increased when ap- plied with 2Hz EA （ P 〈 0.01 ）, while did not change significantly to 100Hz and 2/100 Hz EA. Conclusion EA has immune modulation function and the existence of frequency responsiveness, its efficacy is closely related to the number of stimuli. This will provide the theoretical and practical basis for clinical application of EA.

关 键 词：电针 频率 刺激频度 NK细胞活性 脾指数 T细胞增殖能力 

分 类 号：R2-03[医药卫生—中医学]