傅里叶变换红外光谱实时监测乳腺癌细胞化疗反应性的研究  

Application of FTIR Spectrometry to Monitoring Chemosensitivity of Breast Cancer Cell Line Using 5-FU

作　　者：胡海舰[1,2] 祖超[1] 郭琳[2,3] 胡庆楠 王健生[1] 徐怡庄[2] 吴瑾光[2] 

机构地区：[1]西安交通大学第一附属医院,陕西西安710061 [2]北京大学化学与分子工程学院,北京100871 [3]辽宁中医药大学药学院,辽宁大连116600 [4]登封市人民医院,河南登封452470

出　　处：《光谱学与光谱分析》2013年第11期2935-2939,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50973003);国家自然科学基金青年科学基金项目(30500109);国家(863)计划重大项目(2010AA03A406);科技部平台项目(2005DKA32800);北京市专利基金项目;北京大学先进技术研究院培育项目资助

摘　　要：应用傅里叶变换红外光谱技术监测乳腺癌细胞株MCF-7在化疗药物5氟尿嘧啶(5-FU)干预过程中相应的红外光谱变化,验证其与药物作用的时间和剂量相关性。对数据结果整理发现,在时间相关性方面,随药物作用时间的延长,乳腺癌细胞株MCF-7对脂类的利用率下降,细胞中脂类含量增加,同时肿瘤细胞分裂增殖受阻,细胞内DNA和RNA的含量降低,实验中观察到,48h内,乳腺癌细胞MCF-7的红外光谱中代表脂类变化的峰强比I2 920/I1 460逐渐升高,I1 400/I1 460逐渐降低,代表细胞内核酸变化的峰强比I1 080/I1 550,I1 240/I1 550逐渐降低;在剂量相关性方面,癌细胞红外光谱中峰强比I1 640/I1 550较正常升高,实验中观察到峰强比I1 640/I1 550随药物浓度的增加而呈现逐渐降低的趋势。以上这些变化与乳腺癌细胞在化疗药物干预过程中的生物学改变相符,可作为FTIR实时监测乳腺癌细胞化疗反应性的参考指标。The purpose of the present study was to compare the changes of infrared spectrum of breast cancer cell line MCF-7 treated by 5-fluorouracil with various concentrations and treating time. The results indicate that the relative intensity ratios of /2 9z0/I146o increased in the FTIR spectra of cell line MCF-7 while I14oo/I14oo, I1080/1165o and 11 240/I1550 decreased significantly within forty-eight hours, however, no regular change was observed during 48～72 hours. The relative intensity ratio of I1640/ 1550 decreases along with the increase in the concentration of 5-fluorouraeil. The changes of infrared spectra are consistent with the biochemical changes in breast cancer cell, and it can be the reference designators when assessing curative effect of breast cancer eel1 by FTIR spectrometry.

关 键 词：乳腺癌细胞 5-FU 红外光谱 监测 

分 类 号：O657.3[理学—分析化学]