荧光光谱成像法在板蓝根颗粒质量评价的应用研究  

Research on Application of Fluorescence Spectrum Imaging Method in Banlangen Granule Quality Evaluation

作　　者：何青[1,2] 梁兰 陈振强[2,3] 庞其昌[2,3] 赵静[4] 马骥[5] 

机构地区：[1]广东医学院生物医学工程教研室,东莞广东523808 [2]广东省高等学校光电信息与传感技术重点实验室,广州广东510630 [3]暨南大学理工学院光电工程系,广州广东510630 [4]华南农业大学理学院,广州广东510640 [5]南方医科大学中医药学院,广州广东510515

出　　处：《光谱学与光谱分析》2013年第11期3044-3049,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60908038)资助

摘　　要：板蓝根颗粒的质量直接影响其疗效,选择一种有效的板蓝根颗粒质量检测方法至关重要。传统的检测方法需要对板蓝根样品进行化学预处理,是一种对样品有损的检测方法。为解决这一问题,采用了一种安装液晶滤波器件的荧光光谱成像实验装置对板蓝根样品的荧光光谱特性进行测试,以中国食品药品检定研究院提供的板蓝根标准物质的光谱曲线作为对照品,通过获得不同原材料、不同厂家和不同批次的板蓝根颗粒的荧光特征光谱曲线,并对其进行归一化处理后,与对照品的荧光光谱特征曲线进行对比分析及聚类分析,从整体上探讨了荧光光谱特征曲线与板蓝根颗粒质量的关系。实验结果显示,不同厂家生产的颗粒之间质量存在较大差异,主要体现在荧光的强弱和波峰位置的变化上,通过与标准物质的光谱曲线做进一步对比,可初步判断板蓝根颗粒质量的优劣。这表明了采用荧光光谱成像的方法对板蓝根质量进行检测是一种简便、快捷、无损的检测手段。The curative effect of Banlangen granule is directly affected by its quality, so it is very important to choose a kind of effective method for testing it. The chemical treatment is needed as a pre-treatment for the quality testing by using common method at present, which will change or destroy the sample. For dealing with this problem, in the present paper, a fluorescence spectrum imaging experimental system based on LCTF （liquid crystal tunable filter） is set up to detect the characteristics fluores cence spectrum of Banlangen granules. The characteristic fluorescence spectrum curve of Banlangen standard material provided by Chinese Food Drugs Examination Research Institute is used as a reference substance for a comparison with other Banlangen granules. By normalizing the corresponding characteristic fluorescence spectrum of Banlangen granules of different raw materi als, different manufacturers and different batches, the relationship between the characteristic fluorescence spectrum curve and the quality of Banlangen granule is discussed by using the method of comparative and cluster analysis. The experimental results show that the qualities of Banlangen granules are quite different from different manufacturers, which are basically reflected by the change in the intensity of fluorescence and peak position. All of these indicate that fluorescence spectrum imaging technology is a simple, rapid and nondestructive detection method for the quality control of Banlangen granule.

关 键 词：质量评价 板蓝根颗粒 荧光光谱成像法 

分 类 号：O433[机械工程—光学工程] R282.7[理学—光学]