利用一种新型的组织工程室在体内构建组织工程软骨  

Application of novel silicon bioinert perforated chambers in tissue-engineering of cartilage in vivo

作　　者：姜疆[1] 刘毅[1] 陈黎明[1] 郭树忠[2] 

机构地区：[1]兰州军区兰州总医院全军烧伤整形外科中心,730050 [2]第四军医大学西京医院全军整形外科研究所

出　　处：《中华医学美学美容杂志》2013年第5期372-376,共5页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的 探讨应用一种新型的组织工程室（tissue-engineering chamber）在具有免疫活性的动物体内构建组织工程软骨的可行性.方法 切取新西兰雄性大白兔耳廓软骨,体外构建：（1）软骨细胞-胶原凝胶复合物；（2）软骨细胞-PLGA-胶原凝胶复合物,将复合物置入组织工程室内,再植入实验兔背部皮下；对照组无组织工程室,直接将复合物植入同一实验兔背部皮下.术后8周进行大体形体观察、组织学及免疫组织化学染色、反转录聚合酶链式反应（RT-PCR）检测.结果 大体形态、组织学及免疫组织化学染色、RT PCR检测结果显示,在实验兔背部皮下组织工程室内,构建的软骨细胞-支架复合物均形成了软骨样组织；而直接植入实验兔背部皮下的复合物最终消失或者形成一纤维化的结节.结论 在具有免疫活性的动物体内,应用自主研制的新型多孔硅橡胶组织工程室内微环境成功构建了自体工程化软骨组织,为软骨组织工程技术过渡到临床应用提供了理论依据.Objective To explore the potential applications of a chamber for in vivo tissue engineering,and to establish a novel model for in vivo tissue-engineered cartilage.Methods Auricular chondrocytes were isolated,cultured and identified from the ears cartilages of New Zealand white rab bits; rabbit auricular chondrocytes（RACs） were seeded into the scaffolds：（1） RACs were seeded into collagen gel scaffold; （2） RACs were seeded into PLGA/collagen gel scaffold in vitro,and the compos ites were placed into the chambers and implanted in the donor rabbit.As control groups,the composites were implanted directly subcutaneously in the donor rabbit without using chambers,and the contents were harvested at 8 weeks after implantation.Gross examination,histologic and immunohistochemical staining and RT-PCR test were performed to evaluate the harvested contents.Results Under the same conditions inside the chambers,the contents formed into new cartilage-like tissue by histo logical and immunohistochemical staining and RT-PCR.In contrast,in the control groups without chambers displayed vascular invasion and inflammatory reaction in the subcutaneous layer of skin,which eventually led to fibrous tissue or absorption.Conclusions Cartilage is successfully constructed in an immunocompetent animal model using a bioinert perforated chamber.This method is effective in creating a relatively favorable environment for cartilage regeneration,which may provide a valuable reference for the clinical application of tissue regeneration.

关 键 词：组织工程室 免疫活性动物 组织工程软骨 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]