骨内膜成骨的动物模型  

Rat model of endosteum ossification in fracture healing

作　　者：郑军[1] 董福慧[1] 

机构地区：[1]中国中医研究院骨伤科研究所,北京100700

出　　处：《中国骨伤》2000年第9期522-523,共2页China Journal of Orthopaedics and Traumatology

摘　　要：为了解骨内膜在骨折愈合不同阶段的功能变化及其调节机制 ,设计一个骨折愈合过程中骨内膜成骨的动物模型。方法　将 3月龄雌性SD大鼠 40只随机分成 4组 ,每组 10只 ,在左胫骨造成长 5mm的环形骨膜缺损 ,用骨锉在骨膜缺损区造成长 5mm的骨缺损 ,深达髓腔 ,约显露髓腔的1/ 6。结果　 14天时裸露的髓腔已开始出现骨性关闭的征象。 2 8天时髓腔完全由坚质骨关闭。而各时间点组的骨缺损处填充物均为瘢痕样结缔组织。结论　关闭髓腔的骨质只能来自于骨内膜成骨。该动物模型是成功的。Objective To produce models of endosteum ossification during the course of fracture healing and to study the functional changes of endosteum in the different stages of fracture healing and the regulation mechanism.Methods 40 female SD rats,which were three months old and 280±20g,were chosen and divided randomly into four groups of 10 rats each group.Left tibia of rats was exposed under anaesthesia.A circular periostum defect 5mm in length over the tibia was produced.Then the bone defect of 5mm in length was made by bone file across the periostum defects.The depth of the bone defect was about 1 2mm so that one sixth marrow cavity was exposed.The animals were sacrificed respectively at 7th,14th,21th,28th days after operation.Results The marrow cavity was found closed by osseous tissue at 14 days after operation.It was closed by compact bone at 28 days.However,the bone defect was filled with scar tissue all the time.Conclusion That fibrous tissue within defect cannot be ossified because of lack of periosteum coverage.The new bone that closed the marrow cavity could only be formed by the endosteum.This rat model of endosteum ossification was successful.

关 键 词：生物学模型 骨膜 骨发生 动物模型 骨折 

分 类 号：R683.02[医药卫生—骨科学]