血管紧张素Ⅱ和缬沙坦对血管平滑肌细胞增殖和基质金属蛋白酶9表达的影响  

The effects of angiotensinⅡand valsartan on cell proliferation and expression of MMP-9 in cultured vascular smooth muscle cell

作　　者：周珂[1] 于弋水[1,2] 

机构地区：[1]武汉大学人民医院神经内科,430060 [2]威海市威海卫人民医院神经内科

出　　处：《卒中与神经疾病》2013年第5期269-271,318,共4页Stroke and Nervous Diseases

摘　　要：目的研究血管紧张素Ⅱ（AngⅡ）对大鼠血管平滑肌细胞（VSMC）的增殖及基质金属蛋白酶-9（MMP-9）表达的影响及缬沙坦（Valsartan）的干预作用。方法分离大鼠胸主动脉平滑肌细胞，贴块法原代培养大鼠血管平滑肌细胞，取第5～10代细胞进行实验，随机分成AngⅡ组（A组）、AngⅡ＋Valsartan组（A＋V组）、Valsartan组（V组）及对照组4组，各组细胞分别采用四唑盐（MTT）比色法检测血管平滑肌细胞增殖，逆转录聚合酶链反应和免疫印迹法测定MMP-9mRNA和蛋白的表达水平。结果与对照组比较，AngⅡ组VSMC的增殖率升高；与AngⅡ组比较，AngⅡ＋Valsartan组VSMC的增殖率降低，差异均明显（P〈0．05）。AngⅡ组与对照组比较，MMP-9mRNA和蛋白的表达均升高；AnⅡValsartan组与AngⅡ组比较，MMP_9mRNA和蛋白的表达均降低，差异均显著（P〈O．05）。结论血管紧张素Ⅱ可以诱导血管平滑肌细胞的增殖和基质金属蛋白酶-9mRNA和蛋白的表达增加；血管紧张素Ⅱ1型受体（AT1R）拮抗剂一缬沙坦可抑制此作用，ATR参与介导此过程。[Abstract] Objective To explore the influence of angiotensin Ⅱ and valsartan on vascular smooth muscle cells proliferation and expression of MMP-9. Methods The aorta VSMCs of rats were cultured in vitro by a substrate-attached explanted method. The cultured VSMCs were randomly divided into control group, Ang Ⅱ group（A group）, Ang Ⅱ ＋ Valsartan group（A ＋ V group）and Valsartan group（V group）. The proliferation of the vascular smooth muscle cell were measured by methyl thiazolyl tetrazolium（MTT）. The protein expression of MMP-9 was measured by irnmunohistochemistry staining. MMP-9 mRNA expression was examined by re- verse transcription-polymerase chain reaction （RT-PCR）. Results The VSMCs proliferation in A group was significantly higher than control group （P〈0. 05）. Compared with the A group, the proliferation were signifi- cantly lower in V group （P〈0. 05）. The expression of MMP-9 mRNA and protein in A group were signifi- cantly higher than control group （P〈0. 05）. The expression of MMP-9 mRNA and protein were reduced in A ＋ V group after Valsartan treatment （P〈0. 05）. Conclusions Angiotensin Ⅱ can induce proliferation of vascu- lar smooth muscle cells and expression of matrix metalloproteinases-9, which could be inhibited by ValsartarL Angiotensin Ⅱ type 1 receptor participates in this process.

关 键 词：血管紧张素Ⅱ 血管平滑肌细胞基质金属蛋白酶-9 血管紧张素Ⅱ1型受体 

分 类 号：R972.4[医药卫生—药品] R741[医药卫生—药学]