The potential of trehalose to replace insect hemolymph in artificial media for Trichogramma dendrolimi （Hymenoptera： Trichogrammatidae）  

作　　者：Xin Lu Shi-Chou Han Li-Ying Li Simon Grenier Patrick De Clercq 

机构地区：[1]Guangdong Entomological Institute, Guangzhou, China [2]UMR BF21 INRAIINSA Lyon, France [3]Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium

出　　处：《Insect Science》2013年第5期629-636,共8页昆虫科学（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China Program ＂Optimization of Artificial Diets Composition for Rearing Trichogramma and Reproduction Quality Control （Grant No.31171903）＂.

摘　　要：Insect additives have been shown to improve the value of artificial media for Trichogramma species, but at the same time maintain dependence on parallel cultures of host insects. In the present study, Trichogramma dendrolimi Matsumura was reared in vitro from egg to adult on artificial media with different contents of pupal hemolymph of Chinese oak silkmoth Antheraea pernyi （Guérin-Méneville） and with supplements of distilled water, or of trehalose dissolved in water or in Grace＇s insect medium. The results indicated insect hemolymph was the key component of artificial medium. Developmental parameters, including rates of parasitism, pupation, adult emergence and normal adults, and numbers of produced adults, were increased on media supplemented with trehalose even when the proportion of pupal hemolymph was reduced. Two artificial media, the first containing 30% hemolymph and 10% trehalose in water with 98.9% parasitism rate, 77.7.0% pupation rate, 77.2% emergence rate, 80.0% normal adult rate and 333 produced adults, and the second containing 25% hemolymph and 15% trehalose in Grace＇s insect medium with 97.8% parasitism rate, 91.0% pupation rate, 85.2% emergence rate, 76.1% normal adult rate and 757 produced adults, were believed to hold potential to mass produce T. dendrolimi. The use of trehalose to partially replace pupal hemolymph in artificial medium of this and other Trichogramma species may contribute to a significant reduction in their production cost and may as such help to evade problems related to short supplies of lepidopteran eggs, which currently constitute the main factitious host for the mass rearing of the parasitoids.Insect additives have been shown to improve the value of artificial media for Trichogramma species, but at the same time maintain dependence on parallel cultures of host insects. In the present study, Trichogramma dendrolimi Matsumura was reared in vitro from egg to adult on artificial media with different contents of pupal hemolymph of Chinese oak silkmoth Antheraea pernyi （Guérin-Méneville） and with supplements of distilled water, or of trehalose dissolved in water or in Grace＇s insect medium. The results indicated insect hemolymph was the key component of artificial medium. Developmental parameters, including rates of parasitism, pupation, adult emergence and normal adults, and numbers of produced adults, were increased on media supplemented with trehalose even when the proportion of pupal hemolymph was reduced. Two artificial media, the first containing 30% hemolymph and 10% trehalose in water with 98.9% parasitism rate, 77.7.0% pupation rate, 77.2% emergence rate, 80.0% normal adult rate and 333 produced adults, and the second containing 25% hemolymph and 15% trehalose in Grace＇s insect medium with 97.8% parasitism rate, 91.0% pupation rate, 85.2% emergence rate, 76.1% normal adult rate and 757 produced adults, were believed to hold potential to mass produce T. dendrolimi. The use of trehalose to partially replace pupal hemolymph in artificial medium of this and other Trichogramma species may contribute to a significant reduction in their production cost and may as such help to evade problems related to short supplies of lepidopteran eggs, which currently constitute the main factitious host for the mass rearing of the parasitoids.

关 键 词：artificial medium in vitro rearing TREHALOSE Trichogramma dendrolimi 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治] S567.35[农业科学—植物保护]