Cluster Analysis for Acupoint Specificity of Acupuncture Effect Based on Cerebral Infarction Rat Model  

作　　者：常晓波 樊小农 王舒 孟智宏 杨雪 石学敏 

机构地区：[1]College of Acupuncture-Moxibustion and Tuina,Tianjin University of Traditional Chinese Medicine [2]Critical Research Room of Encephalopathy Acupuncture Therapy [3]The First Hospital Affiliated to Tianjin University of Traditional Chinese Medicine [4]Class Ⅲ Laboratory of Acupuncture Quantity-Effect Relationship Affiliated to the State Administration of Traditional Chinese Medicine [5]Mathematics College of Tianjin Polytechnic University [6]Tianjin Key Laboratory of Acupuncture and Moxibustion Science

出　　处：《Chinese Journal of Integrative Medicine》2013年第11期853-858,共6页中国结合医学杂志（英文版）

基　　金：Supported by the National Program on Key Basic Research Project(973 Program,No.2006CB504504,2010CB530500 and2012 CB 518505)

摘　　要：Objectives： To study the acupoint specificity through the comprehensive evaluation of the acupuncture effect on rat model with the middle cerebral artery occlusion （MCAO）. Methods： Duplicated MCAO model by Zea-longa＇s thread ligation was assessed by Zausinger＇s six-point method, and rats with 1-3 scores were chosen to be grouped. According to the randomized and controlled principle, rats were divided into 6 basic control groups （including normal group, sham group, model control group, model group without intervention, Nimodipine group, lateral-to-Renzhong group） and 6 acupuncture groups [including Neiguan （PC6） group, Weizhong （BL40） group, Chize （LU5） group, Sanyinjiao （SP6） group, Renzhong （DU6） group and non-acupoint group]; in each acupoint or non-acupoint 9 different parameter combinations were respectively set by orthogonal intersection method. There were in total 60 groups, and each group had 12 rats. The rats were treated by acupuncture with the lifting-thrusting manipulation every 12 h, in total 6 times. Indices of neurobehavioral scores, cerebral blood flow, infarction rate, microcirculation, and light microscope, etc. were measured. In order to comprehensively evaluate the acupuncture effect to research the specificity of acupoint, a cluster analysis was made with SPSS17.0 for the comprehensive effect scores of the samples computed according to the comprehensive evaluation models, and then sorting and classification were made for the comprehensive effect scores. Results： In the acupuncture groups every acupoint had different therapeutic effect due to different acupuncture parameter combinations; among the acupuncture groups the orders of curative effect were as followings： Neiguan group was of exceptional result, Weizhong group and Sanyinjiao group were of valid results in upper level, Chize group and Renzhong group were of valid results in lower level, and non-acupoint group was of invalid result; the therapeutic result of acupoint group was superior to non-acupObjectives： To study the acupoint specificity through the comprehensive evaluation of the acupuncture effect on rat model with the middle cerebral artery occlusion （MCAO）. Methods： Duplicated MCAO model by Zea-longa＇s thread ligation was assessed by Zausinger＇s six-point method, and rats with 1-3 scores were chosen to be grouped. According to the randomized and controlled principle, rats were divided into 6 basic control groups （including normal group, sham group, model control group, model group without intervention, Nimodipine group, lateral-to-Renzhong group） and 6 acupuncture groups [including Neiguan （PC6） group, Weizhong （BL40） group, Chize （LU5） group, Sanyinjiao （SP6） group, Renzhong （DU6） group and non-acupoint group]; in each acupoint or non-acupoint 9 different parameter combinations were respectively set by orthogonal intersection method. There were in total 60 groups, and each group had 12 rats. The rats were treated by acupuncture with the lifting-thrusting manipulation every 12 h, in total 6 times. Indices of neurobehavioral scores, cerebral blood flow, infarction rate, microcirculation, and light microscope, etc. were measured. In order to comprehensively evaluate the acupuncture effect to research the specificity of acupoint, a cluster analysis was made with SPSS17.0 for the comprehensive effect scores of the samples computed according to the comprehensive evaluation models, and then sorting and classification were made for the comprehensive effect scores. Results： In the acupuncture groups every acupoint had different therapeutic effect due to different acupuncture parameter combinations; among the acupuncture groups the orders of curative effect were as followings： Neiguan group was of exceptional result, Weizhong group and Sanyinjiao group were of valid results in upper level, Chize group and Renzhong group were of valid results in lower level, and non-acupoint group was of invalid result; the therapeutic result of acupoint group was superior to non-acup

关 键 词：model rats of middle cerebral artery occlusion acupuncture effect comprehensive evaluation acupoint specificity cluster analysis 

分 类 号：R245[医药卫生—针灸推拿学]