济宁青山羊子宫内ERα、ERβ和PR的分布及其mRNA表达的增龄性变化  

Distribution of ERα,ERβ and PR and Expression of Its mRNA in the Uterus of Jining Grey Goats Associated with Aging

作　　者：白淑[1] 同海妮[1] 石运芝[1,2] 黄丽波[1] 侯衍猛[1] 王树迎[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]泰山医学院,泰安271016

出　　处：《畜牧兽医学报》2013年第10期1539-1546,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30871832);山东省自然科学基金(ZR2009DM027);山东省科技攻关项目(2008GG30008018)

摘　　要：旨在探讨济宁青山羊出生后发育过程中雌激素受体（Estrogenreceptor，ERα和ERβ）和孕激素受体（Pro—gesteronereceptor，PR）在子宫的定位及其mRNA的表达规律。利用SABC法检测ERα、ERβ和PR在不同日龄组子宫的分布；以GAPDH为内参基因，qRT～PCR法检测3种mRNA的相对表达量。结果显示，ERα、ERβ和PR阳性物质主要见于腔上皮、腺上皮、基质、血管内皮和平滑肌细胞的胞核中，偶见胞质阳性着色。ERα、ERβ和PR阳性细胞光密度与其mRNA表达趋势基本一致，但ERβ 低于ERα。ERα和E即在出生当天～30日龄处于低水平，60日龄开始上升，90～120日龄达到高峰，以后维持在高水平状态；PR在出生当天较高，而在30日龄处于最低水平，初情期（60日龄）后逐渐升高，并在性成熟（120日龄）和性成熟后（180日龄）出现2个高峰。ERα、ERβ和PR参与调节子宫内膜与肌层的增殖和分化。To detect the location of ERα,ERβ and PR and the expression of ERα,ERβand PR mRNA in the uterus of Jining Grey goats during postnatal development. In the uterus of different group, the SABC methods were used to study the distribution of ERα,ERβ and PR, and the real time fluorescent quantitative PCR were used to research the relative expression of ERα,ERβ and PR mRNA with GAPDH as an internal gene. ERα,ERβ and PR were malinly localized in the nu- cIei of the luminal epithelium, glandular epithelium, stromal cells, endothelium and smooth mus- cle cells of the uterus,and occasionally in the cytoplasm. The trends of the optical density in the immunopositive cells of ERα,ERβ and PR were similar with that of the expression of ERα,ERβ and PR mRNA. But the level of the expression of ERβ was less than that of ERα. From the birth day to the 30th day, the expression of ERα and ERβ remained at a lower level then increasedpeaks were found during the sexual maturity and maturation. ERα,ERβ and PR have an impor- tant role in regulating the proliferation and differentiation of the endometrial and the myometrial.

关 键 词：雌激素受体α、β 孕激素受体 济宁青山羊 子宫 免疫组化 QRT-PCR 

分 类 号：S827[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]