生长分化因子9在牛卵丘卵母细胞复合体体外成熟过程中的表达  

Expression of Growth Differentiation Factor-9 in Bovine COCs during in vitro Maturation

作　　者：欧阳效晴[1] 杨淑青[1] 张春强[1] 栗瑞兰[1] 张通[1] 张家新[1] 

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古自治区动物遗传育种与繁殖重点实验室,呼和浩特010018

出　　处：《畜牧兽医学报》2013年第10期1569-1575,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：公益性行业(农业)科研专项经费(201003061);内蒙古自然科学基金(2010MS0407)

摘　　要：旨在探讨牛卵丘卵母细胞复合体(Cumulus-oocyte complex,COCs)体外成熟过程中生长分化因子9(Growth differentiation factor 9,GDF-9)基因表达和卵丘细胞扩展的关系。运用实时荧光定量PCR技术检测GDF-9基因在牛卵丘卵母细胞复合体体外成熟过程中(0、6、12、18、24和28h)的相对表达变化,同时观察了卵丘细胞扩展情况。结果表明,GDF-9mRNA的表达量在牛卵丘卵母细胞复合体体外成熟过程中呈现一定的规律性变化,随着成熟培养的进行,其表达量逐渐增加,在成熟培养12h时表达量最高,但随后又逐渐降低,在成熟培养24h表达量最低;卵丘细胞在IVM 12h开始出现明显的扩散,而且随着成熟时间的延长,卵丘细胞的扩散程度在不断增加。研究结果表明:高水平的GDF-9mRNA表达与卵丘细胞开始扩散的时间一致,揭示GDF-9基因可能在牛卵丘细胞扩散和卵母细胞体外成熟过程中起着重要的作用。The relationship between GDF-9 gene expression and expansion of COCs during in vitro maturation was investigated in this study. The expression of GDF-9 in bovine COCs during in vitro maturation（IVM）（ 0, 6, 12, 18, 24 and 28 h） were detected by fluorescent quantitative RT-PCR method. The cumulus expansion was also observed. The RT-PCR result showed that GDF-9 mRNA expressed regularly in bovine COCs during in vitro maturation, which increased from 0-12 h culture and attained the highest expression at 12 h maturation culture, but subse- quently decreased gradually and attained the lowest expression at 24 h maturation culture. The cumulus cells of COCs began to expand apparently at 12 h IVM culture, and increased expansion with maturation culture afterwards. The rusults showed that cumulus cells begin to expansion when expression of GDF-9 mRNA was highest in COCs, which suggested that GDF-9 may play essential role in cumulus expansion and oocyte maturation of bovine.

关 键 词：牛 卵丘卵母细胞复合体 GDF-9 体外成熟 

分 类 号：S823[农业科学—畜牧学] S814.8[农业科学—畜牧兽医]