11味中药多糖提取物对不同MHCB-LβⅡ基因型鸡新城疫抗体水平和IFN-γ、IL-4含量的影响  

Effects of Polysaccharide Extracted from 11 Kinds of Traditional Chinese Medicines on ND Antibody Level and IFN-γ,IL-4 Contents in Different MHC B-LβⅡ Genotype Chickens

作　　者：朱晓庆[1] 李效振[1] 乔海博[1] 贾书红[1] 张东升[1] 谷新利[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《畜牧兽医学报》2013年第10期1685-1692,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30960291)

摘　　要：为探讨11味中药多糖提取物对不同MHC B-LβⅡ基因型鸡免疫调节作用的影响,采用PCR-RFLP方法将1日龄雄性良凤青脚麻鸡按不同MHC B-LβⅡ基因型分组,并分别给予高、中、低剂量中药多糖提取物和生理盐水,采用ELISA法测定血清中新城疫抗体水平和IFN-γ、IL-4含量。结果显示:(1)不同剂量中药多糖提取物能提高各MHC B-LβⅡ基因型鸡ND抗体水平和IFN-γ、IL-4含量,且高剂量组中AA基因型个体高于AB、BB基因型个体,中、低剂量组中AB基因型个体高于AA、BB基因型个体;(2)AA基因型个体ND抗体水平和IFN-γ含量在高剂量组高于中、低剂量组,AB、BB基因型个体ND抗体水平和IFN-γ、IL-4含量在中剂量组高于高、低剂量组。结果表明11味中药多糖提取物能不同程度促进各MHC B-LβⅡ基因型鸡ND抗体生成和细胞因子IFN-γ、IL-4的分泌,且在不同MHC B-LβⅡ基因型鸡中的最适免疫调节剂量不同。In order to explore the effects of polysaccharide extracted from 11 kinds of traditional Chinese medicines on immunomodulatory in different MHC B-LβⅡ genotype chickens, PCR- RFLP technique was applied to group one-day-old male liangfeng Green-Feet Ma chickens accord- ing to different MHC B-LβⅡ genotypes, then they were given high, middle and low dose of poly- saccharides extracted from traditional Chinese medicines and saline, respectively. The effects of traditional Chinese medicinal polysaccharide extraction on antibody response kinetics to ND vac- cine and IFN-γ and IL-4 contents in serum were determined by ELISA method. The results showed that： （1） Traditional Chinese medicinal polysaccharide extraction at different dose could improve ND antibody level, IFN-γ and IL-4 contents in each MHC B-LβⅡ genotype chicken, and in high dose traditional Chinese medicinal polysaecharide extraction, AA genotype chicken were higher than that of AB, BB genotype, in middle and low dose traditional Chinese medicinal poly- saccharide extraction, AB genotype were higher than that of AA, BB genotype; （2） The ND an- tibody level and IL-4 contents of AA genotype chicken in high dose traditional Chinese medicinalpolysaccharide extraction were higher than that in middle and low dose group, the ND antibody level, IFN-γ, and IL-4 contents of AB, BB genotype chicken in middle dose traditional Chinese medicinal polysaccharide extraction were higher than that in high and low dose group. These re- sults indicated that the polysaccharide extracted from 11 kinds of traditional Chinese medicines could stimulate ND antibody production and cytokines IFN-γ, IL-4 excretion in different MHC B-LβⅡ genotype chickens, and the optimum immunomodulatory dose were different in each MHC B-LβⅡ genotype chicken.

关 键 词：中药多糖提取物 MHC B-LβⅡ基因 鸡 新城疫抗体水平 细胞因子 

分 类 号：S853.7[农业科学—临床兽医学]