中介素对NF-κB介导的急性心肌梗死大鼠心室重构的影响  

Effects of intermedin on ventricular remodeling after acute myocardial infarction mediated by NF-κB

作　　者：薛莉[1] 李银萍[2] 张辉[3] 

机构地区：[1]宁夏医科大学总医院心脏中心内科,银川750004 [2]宁夏医科大学总医院心脑血管病医院,银川750004 [3]宁夏医科大学研究生院

出　　处：《临床心血管病杂志》2013年第10期789-791,共3页Journal of Clinical Cardiology

基　　金：2010年宁夏科技攻关项目(No:102164129)

摘　　要：目的:探讨中介素对大鼠急性心肌梗死(AMI)后细胞因子表达的影响及对心室重构的作用。方法:结扎大鼠左冠状动脉(冠脉)前降支制备AMI模型,40只动物随机分为对照组、假手术组、AMI组及中介素干预组(IMD组)。各组大鼠通过心脏彩超测定术后28d左室舒张末期内径(LVEDD)及左室收缩末期内径(LVESD)的变化,用Western blot检测胞核内核因子-κB(NF-κB)及胞质内NF-κB抑制蛋白α(IκBα)的表达,荧光定量PCR检测肿瘤坏死因子(TNF)-αmRNA及白细胞介素(IL)-1βmRNA。结果:与假手术组比较,AMI组LVEDD与LVESD均显著升高(均P<0.05);与AMI组比较,IMD组LVEDD与LVESD显著降低(均P<0.01)。与假手术组比较,AMI组和IMD组NF-κB、TNF-αmRNA与IL-1βmRNA表达增加,IκBα表达减少(均P<0.01);与AMI组比较,IMD组NF-κB、IκBα、TNF-αmRNA与IL-1βmRNA表达均减少(均P<0.01)。结论:中介素可能通过抑制梗死组织的NF-κB与IκBα下调TNF-α与IL-1βmRNA表达,从而减轻AMI后的心室重构。Objective：To explore the expression of TNF-α mRNA and IL-1β mRNA in rats with acute myocar- dial infarction （AMI） and the effects of intermediary on ventricular remodeling. Method：Forty AMI rats were ran- domly divided into control group, sham group, AMI group and intermedin intervention group （IMD group）. Left ventricular end diastolic diameter （LVEDD） and left ventricular end systolic diameter （LVESD） were determined by ultrasonic heart beat graph. NF-κB and IκBα were evaluated by Western blot. TNF-α mRNA and IL-1β mRNA were tested by PCR. Result： Compared with sham group, LVEDD and LVESD in AMI group significantly increased （both P〈0.05）. Compared with AMI group, LVEDD and LVESD in IMD group significantly decreased （both P〈0.05）. Compared with sham group, expression of NF-κB, TNF-αmRNA and IL-1β mRNA increased, while IκBa decreased （all P〈0.05 ）. Compared with AMI group, expression of NF-κB, IκBα, TNF-α mRNA and IL-1β mRNA decreased significantly （all P〈0.05）. Conclusion： Intermediary could significantly reduce TNF-α mRNA and IL-1β mRNA expression by decreasing NF-κB level in rat after AMI, and alleviate left ventricular dila- tation after infarction.

关 键 词：心肌梗死 急性 中介素 核因子-ΚB 核因子-κB抑制蛋白α 

分 类 号：R542.2[医药卫生—心血管疾病]