磁场对抑郁模型大鼠行为学的影响  

Effects of magnetic field on the behaviors of depression rat model

作　　者：刘佳[1] 韩丽莎[1] 胡海[1] 马玉亭[1] 计晶晶[2] 巴图腾格斯 

机构地区：[1]包头医学院基础学院,包头014060 [2]包头医学院医学技术学院 [3]包头医学院2009级临床本科六班

出　　处：《中华物理医学与康复杂志》2013年第10期760-762,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：内蒙古自然科学基金（2010MS1116）

摘　　要：目的探讨磁场对慢性不可预知的温和应激（CUMS）模型大鼠行为学的影响及其作用机制。方法将36只Wistar大鼠按随机数字表法分为模型组、磁场组和对照组，每组大鼠12只。对照组不予任何刺激，常规饲养9周；模型组和磁场组大鼠按照CUMS的方法每Et随机给予一种刺激，连续刺激5周。入组5周后，将磁场组鼠置于旋磁机中心，模型组则常规饲养4周。3组大鼠均于入组当天、入组5周后、人组9周后，进行常规、行为学和血尿指标检测。结果入组5周后，对照组的糖水消耗量为（32．4±16．7）ml／24h，与模型组的（25．8±19．7）ml／24h和磁场组的（26．2±18．4）ml／24h比较，差异均有统计学意义（P〈0．05）；人组5周后和入组9周后，模型组和磁场组大鼠的体重均显著低于对照组（P〈0．05），人组9周后，磁场组大鼠体重显著高于模型组（P〈0．05）；3组大鼠存活数量比较采用Fisher确切概率法，3组比较，差异均无统计学意义（P〉0．05）。入组9周后，模型组和磁场组旷场、悬尾实验各项指标与对照组比较，差异均有统计学意义（P〈0．05），磁场组的穿格次数、理毛时间、悬尾不动时间与模型组比较，差异亦有统计学意义（P〈0．05）。入组5周后，磁场组和模型组尿皮质醇水平均高于对照组（P〈0．05）；入组9周后，磁场组血皮质醇含量与模型组比较，差异有统计学意义（P〈0．05）。结论400mT的磁场可减轻慢性应激所致大鼠抑郁状态时行为变化的程度，其机制可能与影响血、尿皮质醇水平有关。Objective To study influences of magnetic field on behavior of depressive rats under chronic unpredictable mild stress, and possible mechanisms under such phenomena. Methods Thirty-six Wistar rats were divided into model group, magnetic field group and control group （n = 12 in each group） according to random number table. The model group and the magnetic field group received CUMS stimulation daily for 5 weeks, while the control group received no any treatment. At the end of the fifth week, rats in the magnetic field group were placed in the center of a magnetic machine （400 mT） , and the model group was raised freely for 4 weeks. The se- verity of depressive behaviors, cortisol levels and contents of C-reactive protein in blood and urine sample were test- ed pre-treatment, at the end of 5 weeks and 9 weeks. Results After 5 weeks, consumption of glucose solution of the control group was higher than the other two groups （ P 〈 0.05 ）. After 5 and 9 weeks, body weights of the mag- netic group and the model group were lower than the control group （P 〈 0.05）. At the end of 9 weeks, perform- ance of the magnetic group as indicated by frequency of grid crossing, grooming time and suspending tail test were improved to a significantly greater extent than the other two groups （P 〈 0.05 ）. In addition, the urine content of cortisol in 24 hours in magnetic field and model groups was higher than the control group at the end of 5 weeks, and the serum level of cortisol in the magnetic field group were significantly higher than the model group at the end of 9 weeks （P 〈 0.05）. Conclusion The 400roT magnetic field can reduce the severity of behavior changes of rats with CUMS-induced depression, which might be attributable to its influence on the cortisol levels in blood and urine.

关 键 词：磁场 慢性应激 行为学 

分 类 号：R312[医药卫生—基础医学]