针刺“足三里”对不同状态大鼠胃运动及中缝大核P物质、胃动素的影响  

Effect of Manual Acupuncture Stimulation of “Zusanli” (ST 36)on Gastric Motility,and SP and Motilin Activities in Gastric Antrum and Nucleus Raphe Magnus in Gastric Hyperactivity and Hypoactivity Rats

作　　者：颜纯钏[1] 彭艳[2] 林亚平[2] 易受乡[2] 陈平 侯艳玲[4] 史冬梅[4] 

机构地区：[1]江西省中医院,南昌330006 [2]湖南中医药大学针灸推拿学院,长沙410000 [3]南昌市中西医结合医院皮肤科,南昌330003 [4]湖南中医药大学研究生院,长沙410000

出　　处：《针刺研究》2013年第5期345-351,共7页Acupuncture Research

基　　金：湖南省教育厅资助课题(No.08C632)

摘　　要：目的:观察针刺"足三里"穴对不同状态大鼠胃蠕动波及胃窦和中缝大核区P物质(SP)、胃动素(MTL)的影响,探讨针刺"足三里"穴调节胃运动与中缝大核的关系。方法:50只SD大鼠随机分成正常组、胃亢组、胃抑组、胃亢+针刺组及胃抑+针刺组,每组10只。采用尾静脉注射胃复安及阿托品复制大鼠胃运动亢进及胃运动抑制模型。造模后针刺大鼠双侧"足三里"穴5min。观察针刺前后胃运动波频率及胃运动波幅的变化,酶联免疫法(ELISA)检测胃窦组织中SP及MTL含量,免疫组织化学法检测中缝大核区SP及MTL的表达。结果:大鼠尾静脉注射胃复安后,与注射前相比,胃运动波幅增高(P<0.05),频率无明显变化(P>0.05);与正常组相比,胃窦组织中的SP、MTL含量上升(P<0.01),中缝大核中SP表达下降,MTL表达上升(P<0.01)。针刺"足三里"可使其胃运动波幅下降(P<0.05),胃窦组织中的SP、MTL含量下降(P<0.01),中缝大核中SP表达上升,MTL表达下降(P<0.05,P<0.01)。大鼠尾静脉注射阿托品后胃运动波幅下降,频率减慢,胃窦组织中的SP、MTL含量降低,中缝大核SP表达增高,MTL表达下降(P<0.05,P<0.01);针刺"足三里"可使其胃运动波幅增高,频率加快,胃窦组织中的SP、MTL含量上升,中缝大核中SP表达下降,MTL表达上升(P<0.05,P<0.01)。结论:针刺"足三里"对胃运动具有明显的双向调节作用。针刺对胃运动的调节可能通过影响中缝大核区SP及MTL表达而发挥作用。Objective To observe the changes of gastric motility and levels of substance P （SP） and motilin （MTL） in the gastric antrum and Nucleus Raphe Magnus （NRM） after manual acupuncture stimulation of ＂Zusanli＂ （ST 36） in gastric hyper activity and hypoactivity rats, so as to analyze the role of NRM in acupuncture mediated adjustment of gastric motility. Methods Fifty SD rats were randomly and equally divided into control, gastric hyperactivity （G-Hypera） model, gastric hypoactivity （G-Hy- pea） model, acupuncture ＋ G-Hypera and acupuncture ＋ G-Hypoa groups （10 rats/group）. G-Hypera model was established by intravenous （tail vein） injection of Maxolon （0.5 mL/200 g） and G-Hypoa model established by intravenous injection of Atropin （0.5 mL/200 g）, respectively. After insertion of acupuncture needles into bilateral ＂Zusanli＂ （ST 36）, the needles were repeatedly manipulated at a frequency of about 2 Hz for 5 min. The intragastric pressure was recorded and analyzed using a physiologi cal signal analysis system. The SP and MTL contents of gastric antrum were measured by ELISA, and SP and MTL immunoactivityof NRM was determined by immunohistochemistry. Results In gastric hyperactivity rats, compared with the control group, the intragastric pressure （not systolic frequency）, SP and MTL contents in the gastric antrum and MTL immunoactivity of NRM were significantly increased （P〈0.05, P〈0.01）, while SP immunoactivity of NRM was apparently decreased in the G-Hypera model group （ P〈0.01 ）. After acupuncture stimulation, the intragastric pressure, gastric SP and MTL contents, and MTL immunoactivity of NRM were all significantly down-regulated （P〈0.05, P〈0.01）, and SP immunoactivity of NRM was markedly up-regulated （P〈0.01） in comparison with the model group. In gastric hypoactivity rats, compared with the control group, the intragastric pressure and gastric systolic frequency, gastric SP and MTL contents, and MTL immuneactivity of NRM were signi

关 键 词：针刺干预 胃运动 胃运动亢进 胃运动抑制P物质 胃动素 中缝大核 

分 类 号：R245.311[医药卫生—针灸推拿学]