电针对坐骨神经慢性缩窄损伤大鼠脊髓N-甲基-D-天冬氨酸受体表达的影响  

Effect of Electroacupuncture Intervention on N-methyl-D-aspartic Acid Receptor Expression in Spinal Cord in Rats with Chronic Constrictive Injury of the Sciatic Nerve

作　　者：闫丽萍[1] 刘跃光[1] 吴辛甜 李守栋[1] 马骋[1] 

机构地区：[1]南京中医药大学江苏省针灸学重点实验室,南京210029 [2]常州市中医院针灸科,常州213003

出　　处：《针刺研究》2013年第5期380-385,共6页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81173329);江苏省自然科学基金基础研究计划(BK 2008458)

摘　　要：目的：通过观察坐骨神经慢性缩窄损伤（CCI）大鼠脊髓相应节段N-甲基-D-天冬氨酸（NMDA）受体表达的变化和电针干预对其的影响，探讨电针干预神经病理性痛的脊髓机制。方法：清洁级雄性SD大鼠，随机分为3组，每组20只。假手术组仅分离坐骨神经，但不进行结扎；模型组采用CCI法制备神经病理性痛模型；电针组于CCI术后11～17d应用韩氏穴位神经刺激仪进行电针干预，针灸美容针刺入大鼠损伤侧“委中”穴与“环跳”穴，刺激时间a0rain，1次jd。免疫组化法测定大鼠脊髓NMDA受体2B亚基（NR2B）的表达；WesterIlblot法测定大鼠脊髓NMDA受体1亚基（NR1）和NR2B蛋白的表达；逆转录一聚合酶链反应法测定大鼠脊髓NR1mRNA和NR2B1TIRNA的表达。结果：与假手术组比较，模型组脊髓NR1蛋白及其mRNA表达量均升高（P〈0．01，P〈0．05）；与模型组比较，电针组脊髓NR1蛋白及其mRNA表达均被逆转（均P〈0．05）。各组脊髓NR2B蛋白及其mRNA的表达差异均无统计学意义（P〉0．05）。结论：电针减轻大鼠神经病理性痛的机制之一，可能与有效地下调脊髓NR1的功能有关。Objective To observe the changes of N-methyI-D-aspartic acid （NMDA） receptor expression of spinal cord after electroacupuncture （EA） intervention in rats with chronic constrictive injury （OCI） of the sciatic nerve so as to reveal the mechanism underlying improvement of neuropathic pain. Methods Sixty male SD rats were randomly divided into sham opera tion （sham） group, CCI model group and EA group in = 20）. CCI model was established by ligature of the right sciatic nerve with a piece of surgical chromic suture. For rats of the sham group, the right sciatic nerve was just isolated without ligature. The rats of the EA group were given with EA stimulation of ＂Weizhong＂ （BL 40） and ＂Huantiao＂ （GB 30） on the injured side at a frequency of 2 Hz, electric current of ] -3 mA for 30 min （increasing 1 mA every 10 min）. The treatment was conducted once a day from the , day to the 20th day after modeling. NMDA receptor 2 B subunit （NR 2 B） protein and mRNA expression levels in the spinal cord were determined by immunohistochemistry, Western blot and reverse transcription （RT）-polymerase chain reaction （PCR）, respectively, and spinal NMDA receptor subunit 1 （NR 1） protein and mRNA expression levels were measured by Western blot and RT-POR,respectively. Results In comparison with the sham group, NR 1 protein and mRNA expression levels of the model group in the spinal cord were considerably upregulated after COl （P〈0.01, P〈0.05）. In comparison with the model group NR 1 protein and mRNA expression levels of the EA group in the spinal cord were evidently down-regulated （ P〈0.05）. No significant changes of NR 2 B protein and mRNA expression after COl and EA stimulation were found after immunohistochemistry, Western blot and RT-POR measurements （ P〉0.05）. Conclusion EA intervention is effective in alleviating neuropathic pain in CCI rats, which may be closely related to its effects in lowering functional activity of NR protein and mRNA in the spinal cord.

关 键 词：电针 神经病理性痛 脊髓 N-甲基-D-天冬氨酸受体 1亚基 2B亚基 

分 类 号：R245.97[医药卫生—针灸推拿学]