不同时辰电针对氯胺酮成瘾大鼠内侧前额叶皮质c-fos、神经元型一氧化氮合酶表达的影响  

Effect of Electroacupuncture Intervention at Different Time-points in A Day on Expression of c-fos and Neuronal Nitric Oxide Synthase in Medial Prefrontal Cortex in Ketamine Addiction Rats

作　　者：吴锋[1] 黄锐[1] 熊克仁[1] 

机构地区：[1]皖南医学院解剖学教研室,芜湖241002

出　　处：《针刺研究》2013年第5期386-392,共7页Acupuncture Research

基　　金：安徽省高等学校省级优秀青年人才基金项目(No.2009SQRZ 182)

摘　　要：目的:观察不同时辰电针"足三里"和"三阴交"穴对氯胺酮成瘾大鼠内侧前额叶皮质(mPFC)c-fos、神经元型一氧化氮合酶(nNOS)表达的影响,探讨电针治疗氯胺酮滥用成瘾的作用机制。方法:SD大鼠随机分为正常对照组、生理盐水组、模型组、电针治疗组,电针治疗组再分为子时(23:00)、卯时(05:00)、午时(11:00)、酉时(17:00)4个亚组,每组8只。每天1次、连续7d经腹腔注射氯胺酮复制氯胺酮成瘾模型。不同时辰电针组在给药7d后分别选取一侧"足三里"和"三阴交"穴给予低频(2Hz)电针治疗,每次30min,连续治疗7d。采用免疫组织化学染色方法检测mPFC内c-fos、nNOS的表达。结果:与正常对照组和生理盐水组相比较,模型组mPFC内c-fos、nNOS免疫反应阳性神经元的表达明显增加(P<0.05)。与模型组相比较,午时、酉时电针组c-fos、nNOS免疫反应阳性神经元的表达明显减少(P<0.05);子时、卯时电针组则无明显变化(P>0.05)。结论:氯胺酮成瘾使mPFC内c-fos、nNOS的表达明显增加,可能促成神经元的适应性改变和损伤;午时、酉时电针"足三里"和"三阴交"穴可明显下调mPFC内c-fos、nNOS的表达,恢复性调节神经元的活动,修复损伤,改善氯胺酮成瘾症状。To observe the effects of electroacupuncture（EA） stimulation of Zusanli（ST 36） and ＂Sanyinjiao＂（SP 6） at different time-points of a day on the expression of c-los and neuronal nitric oxide synthase （nNOS） in the medial prefrontal cortex （mPFC） in rats with ketamine addiction. Methods A total of 56 SD rats were randomized into control group, saline group, model group and EA group. The EA group was further divided into Zi-shi （23：00）, Mao-shi（05 ..00）, Wu-shi （ 11：00） and You-shi （17：00） subgroups （n = 8 in each group or subgroup）. Ketamine addiction model was established by intraperitoneal administra tion of ketamine （100 mg/kg）, once a day for 7 days. EA （2 Hz, 3 V） was applied to unilateral ＂Zusanli＇（ST 36） and ＂Sanyin- jiao＇（SP 6） acupoints for 30 min, once daily for 7 days. The expression of c-los and nNOS in the mPFC was detected by immuno-histochemistry. Results Following EA intervention for 7 days, the animals＇ daily life activities including body stretching, stan ding, jumping, circling, falling on the back and fur-licking in the EA group （Wu-shi and You-shi subgroups） were close to those of the normal control and saline groups, being much better than the model group. Compared with the normal control group and saline group, the expression levels of c-los and nNOS in the mPFO in the model group were significantly upregulated （P〈0.05）. Com pared with the model group, the expression levels of both c-los and nNOS were considerably down-regulated in the Wu-shi （11：00） and You-shi （17： 00） subgroups （P〈0.05）, rather than in the Zi-shi （23： 00） and Mao-shi （05： 00） subgroups （P〈0.05）. Conclusion EA of ST 36 and SP 6 at 11：00 and 17：00 can decrease the expression of c-los and nNOS in the mPFO in ketamine addiction rats, which may contribute to its effects in improving the rats＇ behavior activity.

关 键 词：氯胺酮成瘾 电针 时间医学 内侧前额叶皮质 C-FOS 神经元型一氧化氮合酶 免疫组化 

分 类 号：R245.97[医药卫生—针灸推拿学]