核酸酶催化放大传感体系的设计及其在铅离子比色检测中的应用  

Design of DNA Enzyme Catalytic Amplification-based BiosensingPlatform for Colorimetric Detection of Lead Ions (Ⅱ)

作　　者：高晓霞[1] 贾玉华[1] 杨金凤[1,2] 李继山[1] 杨荣华[1] 

机构地区：[1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,长沙410082 [2]中南大学湘雅医学院附属肿瘤医院,长沙410013

出　　处：《分析化学》2013年第5期670-674,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21075032,21005026,21135001)

摘　　要：基于8-17 DNA酶的催化切割特性和类辣根过氧化物酶DNA酶的氧化还原反应催化特性,发展了一种新型核酸酶催化放大传感体系,并用于Pb2+的比色检测。考察了K+浓度,pH值及反应时间对检测体系的影响。ABTS吸光度变化与Pb2+浓度在5.0～100 nmol/L范围内呈良好的线性关系,检出限为3.0 nmol/L。此外,因Pb2+酶的特异性,本方法对Pb2+具有良好的选择性。Based on the catalytic cleavage of 8- 17 DNA enzyme （DNAzyme） and the catalytic redox of horseradish peroxidase （HRP）-mimicking DNAzyme, a novel DNAzyme catalytic amplification biosensing platform has been proposed for the colorimetric detection of lead ions （ Pb2＋ ）. The effects of K＋ concentration, pH value and incubation time on the detection system were investigated. The system exhibited a dynamic response range for Pb2＋ from 5 to 100 nmol/L with a detection limit of 3 nmol/L. In addition, the selectivity of the sensor for Pb2＋ against other environmentally related metal ions was outstanding.

关 键 词：DNA酶 比色检测 催化放大 生物传感 铅离子 

分 类 号：O657.3[理学—分析化学]