基于大肠杆菌的全细胞微生物传感器的构建及其在急性生物毒性检测中的应用  

Development of a Whole Cells Microbial Biosensor Based on E. coli and Its Application to Acute Biotoxicity Determination

作　　者：钱俊[1,2] 李久铭[2] 只金芳[2] 覃事栋[1] 

机构地区：[1]吉首大学化学化工学院,吉首416000 [2]中国科学院理化技术研究所,北京100190

出　　处：《分析化学》2013年第5期738-743,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.21005067);湖南省自然科学基金(No.11JJ4015);湖南省教育厅优秀青年资助项目(No.12B029)资助

摘　　要：构建了以大肠杆菌为指示生物的全细胞微生物传感器,初步探讨了其在重金属(Cu2+,Zn2+,Pb2+)及其二元联合毒物、农药污染物(莠灭净、乙酰甲胺磷)的急性生物毒性分析中的应用性能。结果表明,基于对数生长后期和稳定期的大肠杆菌微生物传感器具有良好的毒性分析性能。最优实验条件为:E.coli培养16 h,苯醌为电子传递介体,呼吸基质pH 6～8。所测毒物对E.coli的呼吸抑制作用大小顺序为:Cu2+>Zn2+>Pb2+,乙酰甲胺磷>莠灭净,与发光细菌、鱼类、藻类等其它方法检测所得结果一致。这种电化学全细胞生物传感器毒性分析方法具有快速灵敏、成本低廉、操作简单等优点,有望用于水体急性生物毒性的在线监测。E. coli microbial electrode for biosensor was prepared and its performance for acute biotoxicity determination of heavy metals （ Cu2＋, Zn2＋ and Pd2＋ ）, Ametryn and Acephate by single or mixture was studied preliminarily. A measuring condition with E. coli in logarithmic phase （incubated for 16 h）, p-benzoquinone as mediator and pH 6-8 was used as the optimized conditions. The experimental results revealed that the inhibitory effects of toxicants to E. coli＇s respiration were in a decreasing order： Cu2＋〉 Zn2＋〉 Pd2＋, Ametryn〉 Acephate, which was in accordance with the results of other ways, such as the experiments using luminescent bacteria, fish, algae etc. This method is rapid, simple, sensitive, cheap and promising for wastewater monitoring on line.

关 键 词：微生物传感器 大肠杆菌 急性生物毒性 重金属 农药 

分 类 号：X832[环境科学与工程—环境工程]