HPLC测定阿莫西林胶囊中的有效成分  

Determination of Effective Constituent in Amoxicillin Capsule by HPLC

作　　者：陈宏[1] 陈友存[2] 程乐华[1] 汪海燕[1] 王敏[1] 

机构地区：[1]巢湖学院化学化工与生命科学学院,安徽省巢湖市居巢区半汤镇238000 [2]安庆师范学院化学化工学院

出　　处：《光谱实验室》2013年第6期2733-2736,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金资助项目(No.20771006);安徽省高校省级科学研究项目(项目编号:KJ2013B164;KJ2010B126)

摘　　要：建立了HPLC测定市售阿莫西林胶囊中有效成分的方法。采用Agilent C18柱(250mm×4.6mm,5Lm),以十八烷基键合硅胶为固定相,0.05mol·L-1磷酸二氢钾溶液(用2mol·L-1氢氧化钾溶液调节pH值至5.0)(A)-乙氰(B)(97.5∶2.5,V/V)为流动相。流速为1.0mL·min-1,柱温为25℃,检测波长为254nm,进样量为10LL。实验结果表明:回归方程为y=5.27x+2.83,r=0.9995(n=6),线性范围20—500Lg·mL-1,RSD为3.97%,样品中阿莫西林含量为821.3mg/g。此方法具有简单方便、灵敏度高、快速、准确性和重现性好等特点,可用于阿莫西林原料药及其制剂有效成分的质量控制。The mothod of effective constituent in amoxicillin capsule in the markets was established by HPLC. The solution was separated by Agilent C18 column（250mm×4.6mm,5μm） with the stationary phase of oetadecyl bonded silica, potassium dihydrogen phosphate solution （0.05tool/L, in 2mol·L^-1 potassium hydroxide solution to adjust the pH to 5. 0）-aeetonitrile （97.5 ： 2.5, V/V） as mobile phase,flow rate of 1.0mol·min^-1, detection wavelength of 254nm, column temperature of 25C ,and injection volume was 10μL. The regression equation was y=5.27x 2.83,7-= 0. 9995 （n= 6）, and the linear range was from 20 to 500μg with RSD of 3. 97%. The montent of amoxicillin in sample was 821.3mg/g. The method is easy and conveninet, sensitive, fast and accurate with good reproducible, and the way can be used to quality control of the content of amoxicillin and its preparations.

关 键 词：高效液相色谱法 阿莫西林 胶囊 流动相 

分 类 号：O657.72[理学—分析化学]