微波消解-ICP-OES测定大鼠股骨中的28种元素含量  

Determination of the 28 Elements Contents in Rat Femur by Microwave Digestion and ICP-OES

作　　者：唐中涛[1] 乔恩美[1] 胡倩倩[1] 靳二辉[1] 顾有方[1] 李升和[1] 

机构地区：[1]安徽科技学院动物科学学院

出　　处：《光谱实验室》2013年第6期2918-2923,共6页Chinese Journal of Spectroscopy Laboratory

基　　金：教育部科学技术研究重点项目(210095);安徽省自然科学基金(1308085MC50;1208085QC68);安徽科技学院稳定人才基金(ZRC2013354);安徽省高校自然科学研究项目(KJ2012B057)

摘　　要：采用微波消解-电感耦合等离子发射光谱法(ICP-O ES),测定60日龄和90日龄大鼠股骨中A l、As、B、Ba、Be、Bi、Ca、Cd、Co、Cr、Cu、Fe、Ga、K、L i、M g、M n、N a、N i、P、P b、S、Sb、Sr、T i、T l、V和Z n等28种元素含量;股骨采用HNO3-H2O2酸溶体系进行微波消解。结果显示,该方法检出限在0.001—1.689Lg/mL之间;通过添加标准回收实验,回收率在81.51%—106.91%之间;R SD在0.2441%—9.8592%之间;与传统方法相比,该方法具有良好的准确度和精密度,为不同生物样品中元素含量的测定提供了参考。The content of the A1,As,B,Ba,Be,Bi,Ca,Cd,Co,Cr,Cu,Fe,Ga,K,Li,Mg,Mn,Na, Ni, P, Pb, S, Sb, Sr, Ti, T1, V and Zn elements in the rat femur on 60 and 90 days were determined by the microwave digestion-inductively coupled plasma atomic emission spectrometry （ICP-OES）; the femur microwave digested by the HNO3-H202 acid-soluble system. The results demonstrated that the detection limit between 0. 001μg/mL and 1. 689μg/mL; the recovery rate which between 81.51%-106.91 % and RSD which from 0. 2441% to 9. 8592% were determined via standard recovery experiment; this method was better accuracy and precision than traditionary method, and provided a reference for the determination of the element content in the biological samples.

关 键 词：微波消解 电感耦合等离子发射光谱法 大鼠股骨 28种元素 

分 类 号：O657.32[理学—分析化学]