HPLC法测定大鼠盲肠内容物中5-氨基水杨酸的含量  

Determination of 5-Aminosalicylic Acid in Rat Cecal Content Solution by HPLC

作　　者：田睿[1] 刘静[2] 郭锋[2] 谷萌辉[2] 张良珂[2] 

机构地区：[1]重庆医科大学实验教学中心,重庆市渝中区医学院路1号400016 [2]重庆医科大学药学院,重庆市渝中区医学院路1号400016

出　　处：《光谱实验室》2013年第6期3036-3039,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：重庆市自然科学基金(CSTC2012jjA10021);高等学校博士学科点专项科研基金(20125503120003);重庆医科大学大学生科研与创新实验项目(201244;201229;201217);海扶之星学生科研基金项目(XS201309);重庆市教育委员会科学技术研究项目(KJ110323)

摘　　要：建立高效液相色谱法测定5-氨基水杨酸在大鼠盲肠内容物中的含量。色谱柱为依利特C18柱(Hypersil BDS,200mm×4.6mm,5Lm),流动相为磷酸盐缓冲液(含0.01mol·L-1四丁基溴化铵,pH 6.6)∶甲醇=75∶25(V/V),流速1.0mL·min-1,检测波长240nm。实验结果表明大鼠盲肠内容物中的内源性物质不干扰测定;线性范围为1.0—64.0Lg·mL-1(r=0.9994);样品日内日间精密度的RSD均小于2%,回收率在99%—101%之间。本法快捷、灵敏、准确,可用于5-氨基水杨酸大鼠盲肠内容物药物浓度的测定。The aim of this paper is to establish a HPLC method for the determination of 5- aminosaticylic acid in rat cecal content solution. The study was performed on Elite C18 column （Hypersil BDS, 200mm × 4. 6mm, 5μm） with the mobile phase of PBS buffer （containing 0. 01mol- L^-1 tetrabutyl ammonium bromide,pH6.6）-methanol （75 ： 25,V/V） at a flow rate of 1.0mL · min-1. The eluent＇was detected at 240nm. The results of chromatographic method showed a good specificity between analytes and matrix. It showed good linerity over a range of 1. 0-64. 0μg · mL^-1 （r = 0. 9994）. The method recovery was between 99% and 101%,and inter-day and intra-day precision RSDs were all less than 2. 0M. The developed method was fast, sensitive and accurate for determination of 5-aminosalicylic acid in rat cecal content solution.

关 键 词：5-氨基水杨酸 高效液相色谱法 大鼠盲肠内容物 

分 类 号：O657.72[理学—分析化学]