二氯氧锆比色法测定陈皮中的总黄酮  

Determination of Total Flavonoids in Citri Ret iculatae Pericarpium by ZrOCl_2 Col orimetry

作　　者：王建壮[1] 陈楚萍[2] 庞小雄[1] 周晔[1] 潘楚雯 吕华冲[1] 

机构地区：[1]广东药学院药科学院 [2]广东省医学科学院/广东省人民医院药学部,广州市中山二路106号510080

出　　处：《光谱实验室》2013年第6期3134-3137,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：建立陈皮中总黄酮的含量测定方法,以控制其质量。以橙皮苷为对照品,采用ZrOCl2比色法测定陈皮中总黄酮的含量,测定波长为312.5nm。在浓度0.0044—0.022mg/mL范围内,吸光度与浓度呈良好的线性关系(r=0.9999),平均回收率为100.69%,RSD为1.64%(n=6)。本法操作简单,具有良好的稳定性和重现性,适用于陈皮中总黄酮的含量测定和评价。A determination method of total flavonoids in Cirri Reticulatae Pericarpiurn was established to control the quality of Cirri Reticulatae Pericarpium. Hesperidin was chosen as reference substance, and the total flavonoids were measured by ZrOC12 colorimetry with the wavelength of 312.5nm. A good linear relationship between the absorbance and the concentration was found within the concentration range of 0. 0044-0. 022mg/mL （r= 0. 9999）. The average recovery rate was 100.69 %, and RSD was 1.64% （n=6）. This simple method,with good stability and reproducibility, can be used to determine and evaluate the total flavonoids in Cirri Reticulatae Pericarpium.

关 键 词：陈皮 总黄酮 橙皮苷 二氯氧锆比色法 

分 类 号：O657.32[理学—分析化学]