二阶导数光谱法测定溴新斯的明磷脂复合物的复合率  

Determination of Complexation Rate of Neostigmine Bromide-Phospholipid Complex by Second Derivative Spectrometry

作　　者：孙全[1] 陈学梁[1] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心,重庆市渝中区医学院路1号400016

出　　处：《光谱实验室》2013年第6期3264-3267,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：重庆市教育委员会资助项目(KJ090308)

摘　　要：采用二阶导数光谱法测定溴新斯的明磷脂复合物的复合率,以264.2nm处测定振幅D值,测定溴新斯的明磷脂复合物中溴新斯的明的含量,由此计算复合率。结果表明,溴新斯的明在0.10—0.40mg·mL-1浓度范围内线性关系良好,回归方程为y=0.3057x+0.0053,r=0.9992;平均加样回收率为99.86%,RSD为1.16%(n=9)。此方法准确,简便,快速,适用于溴新斯的明磷脂复合物复合率的测定。The complexation rate of neostigmine bromide-phospholipid was determined by second derivative spectrometry. The peak amplitude was determined when the wavelength was at 264.2nm. Then the complexation rate was calculated. The experiment had a good liner relationship range from 0.10 to 0.40mg · mL^-1 for neostigmine bromide content. The regression equation was as follow：y=0. 3057x＋0. 0053 ,r=0. 9992. The average recovery rate was 99.86% ,RSD was 1.16% （n=9）. The method was simple,accurate and rapid. It is suitable for the determination of the complexation rate of neostigmine bromide-phospholipid.

关 键 词：溴新斯的明 磷脂复合物 二阶导数光谱法 复合率 

分 类 号：O657.32[理学—分析化学]