环磷酰胺和地塞米松对小鼠肠道黏膜免疫抑制的比较  

Comparison of the immunosuppressive functions induced by Cyclophosphamide and Dexamethasone in intestinal mucosa in mice

作　　者：李相玲[1] 白莎莎[1] 周联[1] 王青[1] 温如燕[1] 

机构地区：[1]广州中医药大学免疫研究室,广州510405

出　　处：《中国免疫学杂志》2013年第1期39-42,47,共5页Chinese Journal of Immunology

基　　金：广东省教育部产学研究项目(20100907);广州中医药大学创新项目(K0100078)

摘　　要：目的:观察环磷酰胺和地塞米松对小鼠肠道黏膜免疫的抑制作用。方法:筛选地塞米松和环磷酰胺的给药天数和剂量;CCK-8检测肠系膜淋巴结(Mesenteric lymph nodes,MLN)细胞转化增殖;流式细胞术检测MLN及派氏结(Peyer'sPatch,PPs)中T、B淋巴细胞的数量及活化比例;ELISA试剂盒检测肠道sIgA的分泌。结果:地塞米松40 mg/kg和环磷酰胺100 mg/kg腹腔注射给药一次,有效降低小鼠胸腺、脾脏脏器指数和减少派氏结个数,抑制MLN细胞增殖;与正常组相比,地塞米松降低MLN和PPs中T细胞比例,抑制T、B细胞的活化;而环磷酰胺降低MLN和PPs中B细胞的比例,对MLN中B细胞活化有明显的抑制作用;地塞米松和环磷酰胺均降低肠道sIgA的分泌。结论:地塞米松和环磷酰胺皆能对肠道黏膜免疫产生抑制作用,但地塞米松对MLN和PPs中T细胞抑制作用较强,而环磷酰胺对B细胞的抑制作用较强。Objective： To study the immunosuppressive functions induced by Cyclophosphamide （CY） and Dexamethasone （DEX） in intestinal mucosa in vivo in mice. Methods：Firstly screening out the administration dosages and days of DEX and CY; Cell proliferation was detected by CCK-8 in mesenterie lymph nodes （MLN） ; the activation and proportion of T and B lymphocytes were de- tected by Flow Cytometry in MLN and Peyer＇s patch （PPs） ; intestinal slgA was detected by ELISA. Results：DEX （40 mg/kg） and CY （ 100 mg/kg） intraperitoneal injection once could effectively reduce the thymus and spleen Organ Index, the number of PPs, and inhibit lymphocyte proliferation in MLN ; compared with the normal group, DEX reduces the proportion of T cells and inhibits the acti- vation of both T and B cells in MLN and PPs ; CY could reduce the proportion of B cells in the MLN and PPs, and significantly inhibit the activation of B cell in MLN; DEX and CY decreased secretion of intestinal slgA. Conclusion：Both DEX and CY could inhibit in- testinal mueosal immunity significantly, however DEX has strongly inhibited T cells and CY inhibited B cells.

关 键 词：地塞米松 环磷酰胺 肠道黏膜免疫 免疫抑制 

分 类 号：R392.4[医药卫生—免疫学]