用TRAP PCR ELISA及TRAP PCR PAGE测定鼻咽癌端粒酶活性的比较  

COMPARISON BETWEEN TRAP PCR ELISA ANS TRAP PCR PAGE TO DETECT TELOMERASE ACTIVITY IN NASOPHARYNGEAL CARCINOMAS

作　　者：文忠[1] 肖健云[2] 李运斌 唐发清[3] 田勇泉[2] 赵素萍[2] 谭柏林 

机构地区：[1]广州珠海医院耳鼻咽喉科 [2]湖南医科大学湘雅医院耳鼻咽喉科,长沙410008 [3]湘雅医院中心实验室 [4]湘雅医院血液科

出　　处：《中国现代医学杂志》2000年第9期15-17,共3页China Journal of Modern Medicine

基　　金：湖南省科委基金!项目 (编号 98SSY10 0 8( 2 ) );国家自然科学基金的部分资助!(编号 3 9770 93 3 )

摘　　要：比较TRAPPCRELISA及TRAPPCRPAGE检测鼻咽癌端粒酶的活性。方法 :分别采用TRAPPCRELISA及TRAPPCRPAGE检测了 38例鼻咽癌 (NPC)、15例癌旁组织、19例慢性鼻咽炎和鼻咽癌HNE1细胞株、其它 2种癌细胞株及 3种正常细胞的端粒酶表达。并探讨了在不同数量的HNE1细胞中端粒酶表达的灵敏度及HNE1细胞株、5例NPC活检组织经热灭活后端粒酶检测的特异性。结果 :两种方法在NPC、癌旁、慢性鼻咽炎及HNE1细胞株端粒酶表达的阳性率非常接近 ,分别为 84.2 9%和 85 .2 9%、73.3%和6 9.2 %、5 .3%和 12 .1%及 95 .5 %和 91.2 %。ELISA间接定量法能很好地反映不同程度的端粒酶活性状态。在 10 2 HNE1细胞中仍能检测到端粒酶活性 ,而热灭活后测不出其活性。结论 :两种方法均能很好地显示鼻咽部不同组织及细胞中的端粒酶活性状态 ,有较高的灵敏度和特异性。Objective: To compare the telomerase activity of nasopharyngeal carcinomas detected by TRAP PCR ELISA coith that by TRAP PCR PAGE. Methods: Telomerase activity was detected in 38 cases of NPC, 15 cases of PNC adjacent tissues and 19 cases of chronic nasopharyngitis as well as in NHE1 cell lines, two other cancer cell lines and three other normal control cell lines by TRAP PCR ELISA and TRAP PCR PAGE respectively. The sensitivity and specificity of telomerase assay were also analyzed. Results: The positive rates of telomerase assay by the two methods in NPC, NPC adjacent tissues, chronic nasopharyngitis and HNE1 cell lines were 84.2% to 85.2%, 73.3% to 69.2%, 5.3% to 12.1% and 95.5% to 91.2%, respectively. The different activity levels of telomerase were revealed using ELISA indirect quantitative assays. Telomerase activity was detected in 1×102 HNE1 cells, but was not or very little after heat-inactivation. Conclusions: Telomerase activity in different nasopharyngeal biopsies is well revealed when using both methods in which have high telomerase assay sensitivity and specificity also.

关 键 词：鼻咽癌 HNE1细胞株 端粒酶 聚合酶链反应 

分 类 号：R739.630.2[医药卫生—肿瘤]