人芽囊原虫滋养体和包囊的形态学观察  

Observation on morphology of trophozoite and cyst of Blastocystis hominis

作　　者：吴国宏[1] 曹镐禄[1] 苏水莲[1] 黄爱民[1] 谢学斌[1] 胡雅琼[1] 

机构地区：[1]赣南医学院寄生虫学教研室,江西赣州341000

出　　处：《赣南医学院学报》2000年第3期203-205,共3页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：江西省自然科学基金资助

摘　　要：用普通光学显微镜和荧光显微镜对人芽囊原虫的三株滋养体和二株包囊进行了形状、大小和结构的观察。滋养体为生长在LAS双相培养基内 4～ 5天的培养物 ;包囊为带虫者粪便经蒸馏水反复 3次洗涤的沉淀物。均作涂片 ,分别以不染色、碘染色和 1∶10 4 吖啶橙染色 ,镜下识别和鉴定。滋养体以空泡型 (包括二分裂状态的 )占多数 ,其次为变形型 ,颗粒型及其它类型少见。颗粒型为多分裂繁殖体。人芽囊原虫包囊形态较单一 ,都是圆形或卵圆形。人芽囊原虫包囊的光镜照片 ,为首次报道 ;其包囊期在我国也是第一次记载。It had been carried out to observe the shape,size and sdtructure of 3 strain trophozoites and 2 strain cysts of Blastocystis hominis by light and fluorescent microscope.Trophozoites were culture of Blastocystis hominis grown for 4-5d in LAS diphasic medium.Cysts were sediment of carrier feces washed three times in distilled water.Smears of aforsaid protozoa(unstained,iodine stained and acridine orange stained respectively)were studied under light and fluorescent microscope.Trophozoite has diversity of shape and size,among which vacuolar form and binary fission occupied most,amoebiod form is the next,granule form and other form are seldom seen.Granule form is reproductive body by multiple fission.Cysts are round to ovoid,the average diameter of 23 cyst is 5.74μm.The light micrograph of cyst is the first publication in world.The cyst stage is the first report in China.

关 键 词：人芽囊原虫 滋养体 包囊 形态学观察 

分 类 号：R383.3[医药卫生—医学寄生虫学]