高温蛋白酶生产菌BY25不同培养基下产酶差异研究  

Differences in Enzyme Production Induced by Fermentation Media of a Moderate Thermophilic Strain Bacillus subtilis BY25

作　　者：朱泓[1,2,3] 王一明[1] 林先贵[1] 高云才 

机构地区：[1]土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京210008 [2]江苏省中科院植物研究所,南京210016 [3]中国科学院大学,北京100049 [4]红塔集团物资采购中心,云南玉溪653100

出　　处：《土壤》2013年第5期899-904,共6页Soils

基　　金：中科院知识创新项目(KSCX2-EW-N-08);948工程项目(2011-G25);江苏省产学研联合创新资金项目(BY2011166)资助

摘　　要：枯草芽孢杆菌是一种广泛存在于土壤中的产酶细菌,在环境领域也具有广阔的应用前景。本文以一株产高温蛋白酶枯草芽孢杆菌BY25为研究对象,比较添加不同有机氮源的产酶培养基进行发酵时,各处理中枯草芽孢杆菌胞外蛋白酶的组成和产量的差异;并对不同培养基发酵液的粗酶液组分进行酶学性质分析和明胶酶谱检测。结果表明,枯草芽孢杆菌BY25在不同培养基条件下会出现差异性产酶,产酶培养基中添加麸皮有利于中性金属蛋白酶(Npr)的产生,而添加豆饼粉则有利于枯草菌素(Apr)的产生。Bacillus subtilis secret different proteolytic enzymes into culture media during stationary phase.Two major proteases are Subtilisin （Apr） and Metelloproteinase （Npr）,which account for more than 90％ of total activity.Both of them are of important application on industry.In this study,these two proteases induced by different media components,soybean cake and wheat bran were observed.After B.subtilis BY25 fermentation,enzyme property examination showed Apr was induced by soybean cake.Gelatin zymograph was then operated and different bands were revealed among enzyme solutions.However,35 KD Npr protein was observed only in the enzyme solution fermented with wheat bran.

关 键 词：明胶酶谱 产酶培养基 酶学性质 丝氨酸蛋白酶 

分 类 号：Q939.96[生物学—微生物学]