5种杀菌剂对沙打旺黄矮根腐病菌的室内毒力测定  

Efficacy testing of five fungicides against the pathogen of yellow stunt and root rot of Astragalus adsurgens in laboratory

作　　者：李兴龙[1] 李彦忠[1] 

机构地区：[1]草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业科学》2013年第10期1523-1530,共8页Pratacultural Science

基　　金：全国优秀博士学位论文资助项目(201068);教育部博士点基金(20100211110013);国家自然科学基金(31272496公益性行业(农业)科研专项(201303057)

摘　　要：沙打旺黄矮根腐病(Embellisia astragali)是我国近年发现的沙打旺(Astragalus adsurgens)上最严重的病害,其病菌生活在植株体内,至今尚无有关其化学防治的研究报道。本研究选择了百菌清、甲霜灵、代森锰锌、甲基硫灵菌和三唑酮5种杀菌剂,采用菌落生长抑制法和孢子萌发抑制法测定了对该病菌的防治效果。供试杀菌剂对沙打旺埃里砖格孢的菌落生长、孢子萌发和菌丝伸长均产生一定影响,在3个指标测定中EC50由小到大的顺序均为百菌清<甲霜灵<代森锰锌<甲基硫灵菌<三唑酮,其中百菌清在菌落生长、孢子萌发和菌丝伸长试验中的EC50分别为0.028、0.03和0.04 g·L-1,而三唑酮的为0.46、0.48和0.21 g·L-1。Embellisia astragali is a stong and virulent pathogen that develops within milk vetch （Astragalus adsurgens）. So far no research had been conducted on chemical controlling for this serious disease. This study assessed the efficacy of five fungicides on E. astragalus through inhibitory tests for colonal growth, spore germination and hypha elongation. The results showed that five tested fungicides all had reflected the colonal growth, spore germination and hypha elongation of E. astragalus partly. The order of concentration for 50% of maximal effect （EC50） was ehlorothalonil 〈metalaxyl 〈 mancozeb 〈 Thiophanate- methyl〈triadimefon within above 3 tests, the EC50 of chlorothalonil on colonal growth, spore germination andhypha elongation was 0.028, 0.03 and 0.04 g·L^-1 respectively, the EC50 of triadimefon was 0.46, 0.48 and 0.21 g·L^-1 respectively.

关 键 词：杀菌剂 沙打旺埃里砖格孢 抑制作用 菌落生长 孢子萌发 菌丝伸长 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]