中药眩晕Ⅲ号治疗脑动脉粥样硬化机制的实验研究  

Experimental study on the mechanism of traditional Chinese medicine Xuanyun-Ⅲ to treat cerebral atherosclerosi

作　　者：刘式威[1] 刘静[2] 吕霞 梁军[1] 田庆华[1] 刘志刚[1] 尹岭[4] 

机构地区：[1]解放军第211医院神经内科 [2]解放军第211医院特诊科 [3]解放军第65426部队医院 [4]解放军总医院

出　　处：《中国疗养医学》2013年第5期396-398,共3页Chinese Journal of Convalescent Medicine

基　　金：973国家重点基础研究发展计划资助项目(2004CB720302)

摘　　要：目的观察中药眩晕Ⅲ号对脑动脉硬化的治疗作用,并初步探索其作用机制。方法随机将50只雄性SD大鼠分为正常对照组、模型组、辛伐他汀组、眩晕Ⅲ号低剂量组、眩晕Ⅲ号高剂量组。除正常对照组外,在喂食开始时一次性腹腔注射维生素D3(300000IU/kg)加高脂配方饲料喂养方法建立脑动脉硬化大鼠模型。7周后开始灌胃给药,正常对照组和模型组予蒸馏水灌胃。8周后处死大鼠,测各组血脂、脑组织生化指标,并对脑血管作病理检查。结果眩晕Ⅲ号能降低血脂,降低脑组织丙二醛(MDA)含量,改善脑组织超氧化物歧化酶(SOD)活性(P<0.05);模型组脑动脉内膜内皮细胞呈广泛性变性、坏死、脱落,粥样斑块形成。经眩晕Ⅲ号治疗后上述指标均有一定改善。结论眩晕Ⅲ号能改善脑动脉硬化模型大鼠的血脂与氧化能力、减轻脑血管壁的病理损害,这些变化提示眩晕Ⅲ号具有防治脑动脉硬化的作用。Objective To study the therapeutic action and its mechanism of Xuanyun- Ⅲ on cerebral atherosclerosis（AS）.Methods Fifty male adult SD rats were randomly divided into 5 groups：normal control group, model group, Simvastatin group, low-dose Xu- anyun-Ⅲ group, and high-dose Xuanyun- Ⅲ group.Except the normal control group, the rats of the other groups were given an intraperi- toneal injection dose of vitamin D3（300 000 IU/kg）at the beginning of feeding,together with high-fat feedstuff to set up rat models with cerebral arteriosclerosis.Garage administration was conducted after seven weeks,while the normal control group and the model group were given distilled water gavage.After eight weeks, all rats were sacrificed, and the serum lipid and the biochemical criteria such as superoxide dismutase（SOD）,the content of Malondialdehyde（MAD）were detected in the brain tissiies.Pathological examinations of cerebral vascular were performed as well. Results Xuanyun-Ⅲ can reduce serum lipid and the content of Malondialdehyde（MAD）, and improve the SOD activity（P 〈 0.05）.Cerebral endarterium endothelial cells of the rats in the model group presented universal degenera- tion, necrosis, ablation, and atheromatous plaque formed.The above indexes were all improved after the treatment of Xuanyun-RI .Con- clusion Xuanyun-Ⅲ can improve the serum lipid and oxidizability of the rats with cerebral arteriosclerosis, alleviate the pathological lesion of brain blood vessel, which shows that Xuanyun-Ⅲ can prevent and treat cerebral arteriosclerosis.

关 键 词：脑动脉硬化 动物模型 药物干预 大鼠 

分 类 号：R285.5[医药卫生—中药学]