均匀设计法联合Excel优选九分巴布剂的基质配比  

Optimization of Matrix Formulation of Jiufen Cataplasms by Combining Uniform Design and Excel

作　　者：潘娅[1] 周莉玲[2] 

机构地区：[1]华南理工大学化学与化工学院,广州510640 [2]广州中医药大学中药学院,广州510405

出　　处：《中国实验方剂学杂志》2013年第21期1-4,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81001648);中央高校基本科研业务费专项(2012ZZ0045)

摘　　要：目的:优选九分巴布剂的基质配比并考察其体外释放过程。方法:以巴布剂的黏性及释放速率常数为指标,采用均匀设计法考察卡波姆、聚丙烯酸钠、聚乙烯醇、羧甲基纤维素钠及甘油用量对九分巴布剂质量的影响,联合Excel软件对试验数据进行多元回归分析,优化九分巴布剂基质配比,测定九分巴布剂的体外释放度。采用HPLC测定马钱子碱含量,色谱条件为Hypersil BDS C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水-三乙胺-冰醋酸(20∶280∶0.3∶2.4,pH 4.5),流速1 mL·min-1,检测波长254 nm。结果:九分巴布剂的最佳基质配比为卡波姆-聚丙烯酸钠-聚乙烯醇-羧甲基纤维素钠-甘油(1∶1∶1∶1.75∶20)。体外释放试验表明九分巴布剂的体外释药过程符合Higuchi方程(R2=0.902 4)。结论:九分巴布剂基质成型好,体外释药能维持24 h释药。将Excel软件与均匀设计联合的方法简便可行。Objective： To optimize matrix formulation of Jiufen cataplasms and investigate its in vitro release characteristics. Method： With viscosity and in vitro release constant of Jiufen cataplasms as indexes, effects of dosage of carbopol, CMC-Na, sodium polyacrylate, polyvinyl alcohol and glycerol on quality of Jiufen cataplasms were investigated by uniform design, multiple regression of test data was analyzed by Excel, then optimized matrix formulation of Jiufen cataplasms and determined its in vitro release. The content of strychnine was determined by HPLC, chromatographic conditions were as follows： Hypersil BDS C18 column .（4.6 mm x 250 mm, 5 μm） , mobile phase of methanol-water-triethylamine-glacial acetic acid （20 ： 280 ： 0.3 ： 2.4, pH 4.5 ） , flow rate 1 mL -min-1, detection wavelength 254 nm. Result： The best matrix formulation of Jiufen cataplasms was： carbopol-polyacrylate sodium-polyvinyl alcohol-sodium carboxymethylcellulose-glycerol （ 1 ： 1 ： 1 ： 1.75 ： 20）. In vitro release properties of Jiufen cataplasms conformed to Higuchi equation with R2 of 0. 902 4. Conclusion： Jiufen cataplasms had good forming, it could maintenance in vitro release for 24 h. Method combining Excel software and uniform design was simple and feasible.

关 键 词：九分巴布剂 基质配方 均匀设计 马钱子碱 体外释放度试验 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]