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机构地区:[1]太极集团重庆涪陵制药厂有限公司,重庆408000
出 处:《中国实验方剂学杂志》2013年第21期37-40,共4页Chinese Journal of Experimental Traditional Medical Formulae
基 金:中药大品种急支糖浆技术改造项目(2011zx09201-201-18)
摘 要:目的:考察高压炮制对何首乌中大黄素、大黄素甲醚及二苯乙烯苷含量的影响。方法:何首乌采用高压清蒸法炮制,考察不同蒸制时间对何首乌中蒽醌类成分、二苯乙烯苷含量的影响,并观察其在炮制过程中性状变化。采用HPLC测定大黄素、大黄素甲醚含量,色谱条件为Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1%磷酸(80∶20),流速1.0 mL·min-1,检测波长254 nm,柱温30℃,进样量10μL;二苯乙烯苷的HPLC测定条件为Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流速1.0 mL·min-1,流动相乙腈-水梯度洗脱,检测波长320 nm,柱温30℃,进样量10μL。结果:高压蒸制0,1,2,3,4,5,6 h时二苯乙烯苷质量分数(n=2)依次为4.55%,3.54%,2.20%,1.87%,1.52%,1.15%,0.76%。随着蒸制时间的增加,何首乌中结合蒽醌、二苯乙烯苷含量逐渐下降,游离蒽醌含量在一定蒸制时间内随蒸制时间的增加而增多。结论:何首乌作为补益药应用时宜采用高压蒸制4 h炮制品。Objective: To investigate effects of high pressure processing on contents of emodin, physcion and 2, 3, 5, 4'-stilbene glucoside from Polygoni Multiflori Radix. Method: Polygoni Multiflori Radix was processed by high pressure steaming method, effects of different steaming time on contents of anthraquinones and 2, 3, 5, 4'-stilbene glucoside were investigated, and properties change during processing was observed. The contents of emodin and physeion were determined by HPLC, chromatographic conditions were as follows: Diamonsil Cls column (4.6 mm x250 ram, 5μm) , mobile phase of methanol-0.1% phosphoric acid (80: 20) , flow rate 1.0 mL -min-I, detection wavelength 254 nm, column temperature 30 ℃ , injection volume 10 μL. HPLC conditions of 2, 3, 5, 4'-stilbene glucoside were as follows: mobile phase of acetonitrile-water gradient elution, detection wavelength 320 nm; other conditions were the same as these above conditions. Result: The content of 2, 3, 5, 4'-stilbeneglucosidewere4.55%, 3.54%, 2.20%, 1.87%, 1.52%, 1. 15%, 0.76% after high pressure steamed 0, 1, 2, 3, 4, 5, 6 h. By increasing of steaming time, contents of incorporating anthraquinones and 2, 3, 5, 4'-stilbene glucoside from Polygoni Multiflori Radix gradually decreased, but the content of free anthraquinones increased. Conclusion: As tonics applications, Polygoni Multiflori Radix should be heated for four hours under high pressure.
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