岗梅药材的TLC鉴别和HPLC指纹图谱  

TLC Identification and HPLC Fingerprint of Ilex asprella

作　　者：郑夏生[1] 袁玉贤[2] 徐晖[1] 詹若挺[1] 陈蔚文[1] 韩正洲[3] 

机构地区：[1]广州中医药大学中药资源科学与工程研究中心,岭南中药资源教育部重点实验室,广州510006 [2]广州中医药大学中药学院,广州510006 [3]华润三九医药股份有限公司,广东深圳518029

出　　处：《中国实验方剂学杂志》2013年第21期123-126,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目(2012A030100006)

摘　　要：目的：建立岗梅药材的TLC鉴别方法和HPLC指纹图谱。方法：TLC鉴别采用Merck预制硅胶薄层板，以三氯甲烷．甲醇-乙酸乙酯-水-甲酸（20：20：40：10：2）为展开剂，10％硫酸乙醇105qC显色，366am紫外光下检视，HPLC指纹图谱采用PhenomenexSynergiFusionC18柱（4．6mm×250mm，4μm），柱温25℃，乙腈-0．05％磷酸梯度洗脱，流速1mL·min^-1，203nm波长检测；采用国家药典委员会“中药色谱指纹图谱相似度评价系统2004A版”进行相似度计算。结果：岗梅与同属易混淆品毛冬青、铁冬青、满树星等的TLC图有较明显差异，可相互区别。不同来源的岗梅相似度为0．716～0．890，各混淆品与岗梅共有模式的相似度均〈0．630。结论：建立的方法可用于岗梅药材的真伪鉴别，为完善岗梅药材的质量标准提供了借鉴。Objective： To establish a specific TLC method and HPLC fingerprint for the quality evaluation of Ilex asprella. Method： TLC was performed with silica gel of Merck, using CHC13-methanol-EtOAc- water-formic acid （20： 20： 40： 10： 2） as the developing solvent system. After sprayed with 10% H2SO4 ethanolic solution, the plate was heated at 105 ℃ and then observed under UV light at 366 nm. HPLC was performed on a Phenomenex Synergi Fusion Clscolumn （4.6 mm x 250 mm, 4μm） with the column temperature at 25℃. The mobile phase consisting of 0.05% phosphoric acid and acetonitrile was used in gradient elution, with a flow rate of 1.0 mL · min-1. The detection wavelength was set at 203 nm. Result： I. asprella and its adulterants were differentiable for distinctive spots were observed on the TLC spectrum. The similarities of 15 bathes of I. asprella were between 0. 716-0. 890, while that of its adulterants were all below 0. 630, calculated with Similarity Evaluation System for Chromatographic Fingerprint of TCM （ Version 2004 A）. Conclusion ： Both the TLC method and HPLC fingerprint established in this study were suitable for discrimination among I. aspreUa and its adulterants, providing a reliable reference for qualitative evaluation of the medicinal material I. asprella.

关 键 词：岗梅 薄层色谱 鉴别 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]