ARPD精制液中4个指标成分的定量测定  

Content Determination of Four Reference Ingredients in Refined Extract Solution of ARPD

作　　者：陈不尤[1] 欧瑶[1] 李曙光[1] 肖明兵[1] 江枫[1] 周士忠[1] 钱霞[1] 顾红梅[1] 陆亚鹏[2] 

机构地区：[1]南通大学附属医院,江苏南通226001 [2]南通大学航海医学研究所,江苏南通226001

出　　处：《中国实验方剂学杂志》2013年第21期141-144,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省南通市社会发展计划项目(S2008035)

摘　　要：目的:建立抗放射性肺炎方(anti-radiation pneumonia decoction,ARPD)精制液中4个指标成分的定量分析方法。方法:以芍药苷、阿魏酸、咖啡酸、甘草酸为考察指标,采用岛津LC-20AD系列高效液相色谱仪和Kromasil C18色谱柱(4.6mm×250 mm,5μm),流动相乙腈-0.1%磷酸,进行梯度洗脱,流速1.0 mL·min-1,柱温30℃。结果:ARPD精制液中芍药苷、阿魏酸、咖啡酸和甘草酸的质量浓度分别为522,13,12,143 mg·L-1。结论:各指标成分检测方法的稳定性、准确度和回收率良好,可用于ARPD精制液的质量控制。Objective： To establish the analytical method of quality control for the four reference ingredients in anti-radiation pneumonia decoction （ARPD）. Method： Herbaceous peony glucoside, ferulic acid, coffee acid and glycyrrhizic acid were adopted as the reference substances. HPLC method was established using SHIMADZU LC-20AD HPLC system. Kromasil CIs column （4.6 mm x250 mm, 5 μm） was used at 30 ℃. Mobile phase consisted of acetonitrile-0. 1% phosphoric acid was used for gradient elution at a flow rate of 1.0 mL ·min-1 Result： The contents of herbaceous peony glucoside, ferulic acid, coffee acid and glycyrrhizic acid in ARPD refined extract were 522, 13, 12, 143 mg .L-1, respectively. Conclusion： The stability, accuracy and recovery of the methods are good and can be used for the quality control of ARPD refined extract.

关 键 词：抗放射性肺炎方 高效液相色谱 指标成分 

分 类 号：R284.1[医药卫生—中药学]