胃康舒宁促胃癌细胞凋亡机制与线粒体凋亡途径  

Mechanism of Promoting Gastric Cancer Apoptosis by Weikang Shuning and Mitochondrial Apoptosis Pathway

作　　者：蒋时红[1] 吴耀松[1] 刘燕[1] 

机构地区：[1]河南中医学院方剂学科,郑州450046

出　　处：《中国实验方剂学杂志》2013年第21期203-206,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技攻关项目(102102310147)

摘　　要：目的:以线粒体凋亡通路为中心在分子水平上探讨胃康舒宁诱导人胃癌细胞株SGC-7901细胞凋亡的作用机制。方法:将胃癌SGC-7901细胞分别培养于200,400,800 mg·L-1胃康舒宁培养药液中48,72 h后,收集细胞并采用流式细胞术检测胃康舒宁对胃癌细胞线粒体膜电位的影响;利用ELISA方法检测用药后胃癌细胞细胞色素C(Cyt c)的变化情况;制作胃癌细胞爬片后,给予不同浓度水平的胃康舒宁处理胃癌SGC-7901细胞不同时间后采用免疫细胞化学方法检测胃癌细胞中B细胞性淋巴瘤/白血病-2基因(Bcl-2)和Bcl-2基因相关蛋白X(Bax)蛋白的表达情况。结果:胃康舒宁可以使胃癌细胞线粒体膜电位下降(P<0.05),促进细胞色素C释放(P<0.05);胃康舒宁各浓度组均能显著增强胃癌细胞株SGC-7901内Bax蛋白的表达(P<0.05),而显著抑制Bcl-2蛋白的表达(P<0.05),且有一定的浓度依赖性。结论:胃康舒宁在体外诱导胃癌细胞凋亡的机制可能与线粒体凋亡通路有关。Objective: To study the apoptosis-inducing effects of Weikang Shuning in human gastric cancer cells in vitro l.Method: The SGC-7901 cells were cultured in the Weikang Shuning liquid with the concentration is 200,400,800 mg·L-1for 48 and 72 hours,then the cells were collected and the flow cytometry was used to detect the change of mitochondria electric potential.ELISA method was used to detect the change of cytochrome(Cyt c).Prepare of cell slides,the immunocytochemistry method was used to study the effects of Weikang Shuning on the indexes of the induced SGC-7901 including Bcl-2 and Bax.Result: Weikang Shuning could decreased the membrane potential and release of Cyt c of gastric cancer cells(P < 0.05).The expression of Bax was strengthened(P < 0.05),and the expression of Bcl-2 was lessened(P < 0.05).Conclusion: The mechanism of promoting gastric cancer apoptosis by Weikang Shuning in vitro may be associated with the mitochondrial apoptotic pathway.

关 键 词：胃康舒宁方 胃癌细胞凋亡 线粒体凋亡通路 体外实验研究 

分 类 号：R285.5[医药卫生—中药学]