两种煎法所得麻杏石甘汤煎剂对A型流感病毒感染小鼠的治疗作用  

Therapeutic Effect of Maxing Shigan Decoction Proceeded by Two Boilling Methods in Mice Infected with Influenza Avirus

作　　者：郭小鸽[1] 肖子曾[1] 卢芳国[1] 李玲[1] 谭露[1] 杨胜辉[1] 蔡锐[1] 朱应武[1] 张波[1] 向琴[1] 

机构地区：[1]湖南中医药大学,长沙410208

出　　处：《中国实验方剂学杂志》2013年第21期227-231,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072751);湖南省科技计划项目(2009SK3120);国家"863计划"专项课题(2009AA10Z403);湖南省卫生厅科研课题(2010014);湖南省高校科技创新团队<感染性疾病中医药防治研究>建设项目(15);湖南省省级精品课程<免疫学与病原生物学>建设项目(48);湖南省重点学科<中西医结合基础>建设项目(100602);湖南中医药大学重点学科<病原生物学>建设项目(1);湖南省学位与研究生教育教学改革研究项目(JG2011B030);湖南省自然科学基金项目(13JJ6062);湖南省教育厅科学研究重点项目(13A066)

摘　　要：目的:比较研究麻黄先煎和4味药同煎所得麻杏石甘汤煎剂对A型流感病毒感染小鼠的治疗效果。方法:采用鼻腔接种法建立A型流感病毒小鼠肺部感染模型,实验设正常对照组、模型对照组、麻黄先煎组、4味药同煎组、奥司他韦组,经灌胃连续给药7 d。分别于感染后治疗的第4,7天检测小鼠体重,第7天,常规检测肺质量指数;光学显微镜技术观察肺病理变化,透射电子显微镜技术观察肺组织超微结构。结果:①体重:感染后治疗4,7 d,与正常对照组比较,模型对照组体重显著下降(P<0.05);治疗7 d,与模型对照组比较,奥司他韦组、麻黄先煎组体重显著增加(P<0.01或P<0.05)。②肺指数:与正常对照组比较,模型对照组肺指数显著增加(P<0.01);与模型对照组比较,各治疗组肺指数明显降低(P<0.01或P<0.05),其中麻黄先煎组与模型对照组比较,较显著性差异(P<0.01),高于4味药同煎组与模型对照组比较的差异性(P<0.05)。③肺部病理变化:与正常对照组比较,模型对照组肺正常组织结构消失,肺泡内有大量炎症细胞浸润。与模型对照组比较,各治疗组肺组织病理变化得到改善,其中奥司他韦组、麻黄先煎组肺部病变程度较4味药同煎组轻。④肺组织超微结构:与正常对照组比较,模型对照组小鼠肺组织超微结构受到破坏,表现为核膜与质膜模糊不清,细胞器减少,线粒体嵴减少或消失,内质网出现空泡样病变,胞浆内有大量病毒颗粒,各治疗组肺组织细胞超微结构得到改善,其中奥司他韦组、麻黄先煎组小鼠肺组织超微结构较4味药同煎组完整。结论:麻黄先煎煎剂抗A型流感病毒作用优于4味药同煎煎剂。Objective： A comparative study of two boiling method （ephedra first boiling and four herbs with boiling） the proceeds Maxin Shigan decoction of influenza A virus infection in mice. Method： Nasal inoculation method to establish the type A influenza virus lung infection in mice model, experimental set normal comparison group, model comparison group, ephedra first boiling group, four herbs with boiling group, osehamivir group, 7 days of continuous given medicines by intragastric. Respectively, 4 days and 7 days after infection to detect the body weigh of mice, on the 7th day, routine testing lung weight index; observation of lungchanges by optical microscopy techniques, lung tissues were observed ultrastructure by transmission electron microscopy techniques. Result： （1） Body weight： 4 days and 7 days after infection, compared with the normalcomparison group, model comparison group significant weight loss （P 〈 0.05） , 7 days after therapy, compared with the model comparison group, Oseltamivir group, ephedra first boilinggroup significant weight gain （P 〈 0.01 or P 〈 0.05）. （2） Lung index： compared with the normal comparison group, the mouse lung index of model comparison group increased significantly （P 〈 0.01 ）. Compared with the model comparison group, lung index was significantly reduced in all treatment groups （ P 〈 0.01 or P 〈 0.05 ）. The significant difference （ P 〈 0.01 ） about of the ephedra first boiling group compared with the model comparison group is higher than thefour herbs with boiling group compared with the model comparison group on the significance （P 〈 0.05 ）. （3）Lung pathological changes ： compared with the normal comparison group, the mouse lungs normal tissue structure disappeared, the alveolar inflammatory cell infiltration. Compared with the model comparison group, lung tissue of all treatment groups pathological change significantly improvely, among oseltamivir group and ephedra first boiling group, the level of lung disease

关 键 词：麻杏石甘汤 煎法 A型流感病毒 肺指数 病理变化 超微结构 

分 类 号：R285.5[医药卫生—中药学]