小半夏汤对豚鼠离体肠管运动的影响  

Effects of XiaoBanXia Decoction on Movement of Isolated Ileum in Guinea Pig

作　　者：林艳艳[1] 聂克[1] 卢燕[1] 张勇[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2013年第21期245-249,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省高等学校科技计划项目(J09LF07);教育部留学回国人员科研启动基金(教外司留[2007]1108号)

摘　　要：目的:观察小半夏汤对豚鼠离体肠管收缩的影响,初步探讨其止吐的作用机制。方法:利用离体浴槽,观察小半夏汤[(生药,下同)0,0.1,0.25,0.5,1,2.5,5,10,20,40 g·L-1]对豚鼠离体肠管自主收缩平均张力、振幅和频率的影响;以呕吐相关受体激动剂乙酰胆碱(Ach)、组胺(His)、五羟色胺(5-HT)、多巴胺(DA)为工具药,观察小半夏汤0,2,5,10g·L-1对各工具药致豚鼠离体肠管收缩平均张力、振幅、频率和最大张力的影响。结果:小半夏汤能剂量依赖性地抑制豚鼠离体肠管自主收缩,降低其收缩平均张力、振幅和频率(与空白对照组相比,P<0.05或P<0.01);先加Ach,His,5-HT再加小半夏汤2,5,10 g·L-1或小半夏汤2,5,10 g·L-1预处理均能明显抑制3种工具药所致肠管收缩,减少肠管收缩平均张力、振幅、频率和最大张力(分别与给小半夏汤前和不给小半夏汤相比,P<0.05或P<0.01);小半夏汤能够增大DA致肠管收缩平均张力、振幅和频率(分别与给小半夏汤前和不给小半夏汤相比,P<0.05或P<0.01),但对肠管收缩最小张力无明显影响。结论:小半夏汤能够抑制豚鼠离体肠管自主收缩,其作用机制可能是通过阻断M受体、H1受体、5-HT受体和D2受体等呕吐相关受体而介导,或直接作用于肠平滑肌而起作用,其止呕机制有待进一步研究。Objective ： To observe the effect of Xiaobanxia decoction on the movement of isolated ileum in guinea pig and analyze the mechanism of anti-emetic effect of Xiaobanxia decoction （XBXD）. Method： The effects of XBXD （0, 0. 1, 0.25, 0.5, 1, 2.5, 5, 10, 20, 40 g.L -~） on ileum spontaneous contraction tension, amplitude and frequency were. investigated in organ bath. Emesis-relating agonists acetylcholine （Ach） , histamine （His）, 5-hydroxytryptamine （5-HT）, dopamine （DA） were used as tool drugs, the inhibitory effect of XBXD （0, 2, 5, 10 g·L^-1） on contraction tension, amplitude and frequency of isolated ileum in guinea pig were observed. Result： XBXD could dose-dependently inhibit ileum spontaneous contractions, reducing the average tension, amplitude, frequency （P 〈0.05 or P 〈0.01 vs control）. Ach, His and 5-HT could stimulate ileum contraction, enhance the tension, amplitude and frequency. All the three doses （10, 5, 2 g ·L^-1） of XBXD could suppress the ileum contraction stimulated by above three tool drugs, significantly reduced the contraction tension, amplitude and frequency （P〈0.05 or P〈0. 01 vs before XBXD or XBXD 0 g·L^-1）. DA could inhibit the spontaneous contraction, the middle and small dose （5, 2 g ·L^-1 ） of XBXD could antagonize the inhibitory effect of DA, and enhance the contraction tension, amplitude and frequency （P 〈 0.05 or P 〈 0.01 vs before XBXD or XBXD 0 g ·L^-1 ）. Conclusion ： XBXD can inhibit spontaneous contractile activity of ileum in guinea pig, the mechanism may be related to blocking M, H1, 5-HT and D2receptors or directly suppressing ileum smooth muscle. The mechanism of anti-emetic effect of XBXD needs further study.

关 键 词：小半夏汤 离体肠管 M受体 H1受体 5-HT受体 D2受体 

分 类 号：R285.5[医药卫生—中药学]