肺痹通方对肺纤维化大鼠胶原蛋白及相关因子的影响  

Effect of Feibitong Formula on the Collagen and Related Factors in Rats with Pulmonary Fibrosis

作　　者：樊茂蓉[1] 李艳艳[2] 张琼[1] 王书臣[1] 张燕萍[1] 张旭丽[1] 

机构地区：[1]中国中医科学院西苑医院,北京市海淀区西苑操场1号100091 [2]中国科学院半导体研究所

出　　处：《中医杂志》2013年第21期1859-1862,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30901948);中国中医科学院西苑医院院内课题(xyky-mp-05)

摘　　要：目的探讨肺痹通方治疗肺纤维化的作用机制。方法将60只SD大鼠随机分为正常对照组、模型组、激素组、肺痹通低剂量组及肺痹通高剂量组各12只,除正常对照组外其余各组大鼠气管内注射博来霉素建立肺纤维化大鼠模型,造模后28天激素组给予强的松片3mg/(kg·d)灌胃,肺痹通低、高剂量组分别给予肺痹通方10g/(kg·d)、20g/(kg·d)灌胃,正常对照组和模型组给予2ml/d蒸馏水灌胃,连续21天。观察各组大鼠肺组织病理形态学,检测血清层粘连蛋白(LN)、转化生长因子-β1(TGF-β1)、谷胱甘肽(GSH)及肺组织Ⅰ型胶原、Ⅲ型胶原、基质金属蛋白酶(MMP-1)和其组织抑制因子(TIMP-1)水平的变化。结果与正常对照组比较,模型组大鼠血清LN、TGF-β1及肺组织MMP-1、TIMP-1、Ⅰ型胶原、Ⅲ型胶原水平升高(P<0.05)。激素组与肺痹通高剂量组LN水平低于模型组(P<0.05);各组大鼠血清GSH水平比较差异均无统计学意义(P>0.05)。激素组及肺痹通高、低剂量组MMP-1、TIMP-1、I型胶原水平均较模型组显著降低(P<0.05);肺痹通高剂量组MMP-1水平较肺痹通低剂量组明显升高(P<0.05);激素组较肺痹通低剂量组TIMP-1水平明显降低(P<0.05)。结论肺痹通方可能通过调节MMPs/TIMPs比例失衡,减少LN以及Ⅰ、Ⅲ型胶原纤维的表达,从而延缓肺纤维化进程。Objective To research the mechanism of Feibitong Formula （formula to treat pulmonary fibrosis） for pulmonary fibrosis. Methods Sixty rats were randomized into normal control group, model group, hormone group, Feibitong low-dose group and Feibitong high-dose group, with 12 in each. The pulmonary fibrosis models were established by intratracheal iniection of hieomycin in all groups hut normal control group. The hormone group, Feihitong low-dose group, Feihitong high-dose group was gavaged with 3 mg/kg Prednisone Tablets, 10 g/kg Feibitong Formula, 20 g/kg Feibitong Formula respectively, normal control group and model group was given 2 ml distilled water 28 days after modeling. The intragastric administration lasted for 21 days. The pathological form in lung tissue was observed. The levels of laminin （LN）, transforming growth factor-β1 （TGF-β1）, glutathione （GSH）, type Ⅰ and type Ⅲ collagens in lung tissues, matrix metalloproteinase-1 （MMP-1） and tissue inhibitor of metalloproteinase-1 （TIMP-1） were detected. Results Comparing with the normal control group, the levels of LN, TGF-β1, MMP-1, TIMP-1, Ⅰ and Ⅲ type collagens in lung tissues in the model group were significantly increased （P〈.0.05）. Comparing with the model group, the LN levels were significantly decreased in the hormone group and Feihitong high-dose group （P〈0.05）. There was no significant difference between groups in the GSH level （P〉0.05）. Comparing with the model group, the levels of MMP-1, TIMP-1 and type Ⅰ collagen were significantly decreased in the hormone group and Feibitong high-dose and low-dose groups （P〈0.05）. The MMP-1 level in the Feibitong high-dose group was significantly higher than that in the Feibitong low-dose group （P〈0.05）. The TIMP-1 level in the hormone group was significantly lower than that in the Feibitong low-dose group （P〈0.05）. Conclusion Feibitong Formula can delay the process of pulmonary fibrosis by regulating the imbalance of MMPs/TIMPs and reducing

关 键 词：肺痹通方 肺纤维化 胶原蛋白 基质金属蛋白酶 层粘连蛋白 

分 类 号：R285.5[医药卫生—中药学]